Development of novel method for visualizing fish spermatogonia using transferrin receptor

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K19138
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Ichida Kensuke 東京海洋大学, 学術研究院, 助教 (70882637)
• Project Period (FY): 2021-07-09 – 2024-03-31
• Keywords: トランスフェリン受容体 / 細胞表面抗原

Outline of Final Research Achievements

Our laboratory previously have been established surrogate broodstock technology and it is expected to be aplied to various kinds of species and situations. In this technology, the small portions of spermatogonial population can be incorporated into the recipient gonads. therefore, it is important to vizualize, isolate and trace the donor cells through the series of experimental process. In this study, we have tried to establish the live cell staining techniques of spermatogonia by transferrin or iron, which are labeled with fluorescent substrate, via binding and endocytosis of transferrin receptor1.

Free Research Field

魚類発生工学

Academic Significance and Societal Importance of the Research Achievements

トランスフェリン受容体を利用した精原細胞の可視化技術は全く報告がない挑戦的な試みとなった。蛍光トランスフェリンを用いた生体染色では、トランスフェリン特異抗体を用いた染色パターンとの一致は見られなかったが、特異抗体を用いたニジマス精原細胞の細胞操作は可能となり、代理親魚技術における有用な技術の１つになる可能性が示唆された。