2023 Fiscal Year Final Research Report
2D Ion Channel Density and Single Molecule Activity: Unraveling a Novel Modality of Membrane Protein Regulation
| Project/Area Number |
21K19212
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
|
|---|
| Research Institution | University of Fukui |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2021-07-09 – 2024-03-31
|
| Keywords | チャネル / 脂質2重膜 / 離合集散 / 電気生理 / 蛍光 |
|---|
| Outline of Final Research Achievements |
The quantitative effects of the aggregation and dispersion of membrane proteins on the cell membrane on their activity have not been clearly elucidated. In this study, we aimed to develop an experimental method that allows functional analysis while controlling the two-dimensional density of membrane proteins using our unique lipid bilayer formation technique, the CBB method. In the experiments, the ion channel protein KcsA was fluorescently labeled, and we analyzed differences in the aggregation states of KcsA within the membrane and the changes in the behavior of KcsA upon manipulation of the membrane area. Through these analyses, we were able to obtain fundamental data for establishing a two-dimensional density control method for membrane proteins.
|
| Free Research Field |
脂質2重膜を使ったチャネルタンパク質の機能解析
|
| Academic Significance and Societal Importance of the Research Achievements |
細胞膜上での膜タンパク質の離合集散を実験的に制御できれば、これまで定量的に解析されていなかった集合状態が活性に与える影響を検討することができる。そのための実験手法を、独自の脂質二重膜操作技術を用いて開発することを目指した本研究は、タンパク質活性の新たな制御様式を解明するという学術的課題に挑むものである。膜タンパク質は、細胞の物質輸送や情報伝達の中心的な役割を果たす重要な分子である。離合集散による制御の仕組みを考慮に入れて医薬品の膜タンパク質に対する作用機序を再評価することで、より精度の高い医薬品設計が可能になるため、社会的意義も大きい。
|
