2022 Fiscal Year Final Research Report
Development of new probes for visualization of subcellular distribution of lipids
| Project/Area Number |
21K19231
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2021-07-09 – 2023-03-31
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| Keywords | 膜流動性 / スフィンゴミエリン / コレステロール / 生体膜 / ラフト |
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| Outline of Final Research Achievements |
Biological membranes are composed of diverse lipid molecular species, and the spatiotemporal heterogeneous distribution of these lipid species is thought to be responsible for diverse intracellular functions. In this research project, I aimed to develop tools to visualise such diversity of plasma membrane lipids and the physical properties of plasma membranes formed as admixtures of diverse lipid species. Pyrene is a solvatochromic dye and allows visualisation of the order of the membrane. However, pyrene backbone dyes are highly membrane permeable, so the signal at the plasma membrane is relatively low. In this project, I developed a probe to visualise the heterogeneity of plasma membrane properties by modifying the fluorescent solvatochromic dye of the pyrene dye and selectively localising it to the plasma membrane.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
生体膜は多様な脂質分子種によって構成されており、これらの脂質種が時空間的に不均一に分布することが、多様な細胞内機能に関わると考えられている。人工脂質二重膜において、スフィンゴ脂質やコレステロールは、秩序性の高い膜領域を形成し、他の膜領域と相分離する性質を示すが、生細胞の形質膜の物性が不均一であるかについては、解析手法が限られるため未解明である。本研究では、周囲の膜の物性に依存して異なる波長の蛍光を発する環境依存性プローブを改変し、生体膜の不均一性の可視化するプローブの開発を行った。
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