2022 Fiscal Year Final Research Report
A technical platform for optical manipulation of synaptic molecules
| Project/Area Number |
21K19311
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 46:Neuroscience and related fields
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| Research Institution | Mie University |
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Principal Investigator |
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| Project Period (FY) |
2021-07-09 – 2023-03-31
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| Keywords | 光操作 |
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| Outline of Final Research Achievements |
The CALI method is a molecular function inactivation method using photosensitizers that release reactive oxygen species in a light-irradiation-dependent manner. It is currently extremely difficult to develop CALI methods for various neurotransmitter receptors one after another. In this study, we applied our new method for rapid optimization of CALI efficiency (high-throughput CALI method) to various molecules and successfully demonstrated the development of high-throughput CALI methods for major membrane proteins.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
従来のCALI法は、分子ごとにCALIが可能な抗体をスクリーニングする必要等の制約があるため、様々な分子に対するCALI法を次々に開発することは極めて困難であった。主要な受容体に対するCALI法をハイスループットに開発できることが実証された本技術により、将来的にはシナプス～脳領域レベルのマルチスケールに対応したシナプス分子解析のための技術プラットフォームの確立が期待できる。
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