Epigenetic regulation as novel approach for organ regeneration - Learning from the stress response mechanism

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K19533
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 55:Surgery of the organs maintaining homeostasis and related fields
• Research Institution: Nagasaki University
• Principal Investigator: LI Tao-Sheng 長崎大学, 原爆後障害医療研究所, 教授 (50379997)
• Co-Investigator(Kenkyū-buntansha): 後藤 信治 長崎大学, 原爆後障害医療研究所, 助教 (50186889)
• Project Period (FY): 2021-07-09 – 2024-03-31
• Keywords: イモリ / エピジェネティック制御 / ストレス応答 / 再生 / 癌 / 放射線傷害

Outline of Final Research Achievements

We evaluated the differences in the expression of metabolic and epigenetic-related factors in lung and heart tissues of healthy adult mice and newts under different oxygen conditions. The results showed that 8% oxygen (hypoxia) stimulation significantly increased the expression of Dnmt3α, Mbd2, Mbd3, and Hdac2 in newt lung tissue, and significantly decreased the expression of Dnmt1 and Dnmt3α in mouse lung tissue. We also succeeded in efficiently expanding primary tissue cells from newts using our original method. These primary tissue cells from newts can be maintained for long periods without senescence, and are sensitively respond to radiation and oxidative stress stimuli.

Free Research Field

生物学

Academic Significance and Societal Importance of the Research Achievements

本研究で、イモリとマウスは酸素濃度変化に対する応答機構が異なることを判明できた。また、我々の改良した方法でイモリ組織細胞を長期培養増幅に成功し、イモリの組織細胞をin vitro実験に提供することが可能となった。これらの研究成果は、イモリに秘めている生物学的特性に関する分子機構の解明に繋がり、臓器再生、発癌予防、及び放射線傷害軽減などにブレークスール成果を生み出すことに期待できる。