Regulation of nurturing by osteocytes

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K19555
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: HAYASHI Mikihito 東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (50581914)
• Project Period (FY): 2021-07-09 – 2023-03-31
• Keywords: 骨細胞

Outline of Final Research Achievements

In recent years, it has become clear that osteocytes are involved in the regulation of other organs, but the mechanisms of their regulation remain largely unknown. In this study, osteocytes were inducibly deleted using newly generated osteocyte-specific Cre-expressing mice. Although effects on maternal behavior were observed in preliminary experiments, no significant differences were observed in the retrieval test, Fos expression in the region including the MPOA, and maternal behavioral abnormalities when N was increased. In addition, in vivo metabolic labeling using osteocyte-specific Cre mice identified osteocyte-derived molecules in the brain, including cytokines and growth factors.

Free Research Field

骨生物学

Academic Significance and Societal Importance of the Research Achievements

これまでに、骨細胞や骨芽細胞が産生し他臓器に作用する複数の分子が同定されているが、in vivoでの網羅的なスクリーニングによるものではなく、in vitroでの発現プロファイルや機能既知分子であることに依るものであった。本研究では真に骨細胞特異的に、in vivoでしかも代謝ラベリングによるタンパク質レベルでの特定臓器における網羅的・誘導性発現解析・同定を行うことができる方法を確立した。このことにより、さらなる骨細胞による他臓器制御メカニズムに迫ることが可能となった。