2022 Fiscal Year Final Research Report
Exploratory research for prediction of severity of respiratory virus infection using nasal secretory vesicles.
Project/Area Number |
21K19574
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Project Period (FY) |
2021-07-09 – 2023-03-31
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Keywords | 粘膜免疫 / 自然免疫 / RSウイルス |
Outline of Final Research Achievements |
Along with developing therapeutic and rapid diagnostic methods for respiratory viral infections, we analyze secretory extracellular vesicles (EVs) during infection to obtain non-invasive and condensed information from the living body. We investigated whether the contents in EVs could serve as severity-specific biomarkers. A preliminary study was conducted to identify the specific EV contents induced during infection, but the marker for the EV contents could not be detected as a protein. Primary cultured pediatric adenoid epithelial cells were infected with GFP-RSV, and RNA- sequence was performed. As a result, the infected cells showed increased expression of genes encoding secretory proteins and noncoding RNAs, including multiple microRNAs. Further analysis of host proteins that bind to RSV-NS1 suggested binding to multiple host proteins that may be released into vesicles. These findings indicate that RSV-NS1 is extracellularly released together with host proteins.
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Free Research Field |
粘膜自然免疫
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Academic Significance and Societal Importance of the Research Achievements |
呼吸器感染性ウイルスは治療薬開発やワクチン開発が進んでいるが、感染力の強さや罹患率の高さからは、適切な介入因子を探索することも治療薬開発やワクチン開発とともに非常に重要で臨床的、社会的意義が大きい。本研究の遂行によって、これまで知られていなかったウイルスタンパク質単独での放出とバイオマーカーとしての可能性を示すことができ、挑戦的萌芽研究遂行の波及効果として罹患率の高い感染症における有用な介入因子のさらなる解析を進める予定である。
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