2022 Fiscal Year Final Research Report
Development of non-invasive environmental exposome analysis using epidermal keratins
Project/Area Number |
21K19838
|
Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 63:Environmental analyses and evaluation and related fields
|
Research Institution | Tohoku University |
Principal Investigator |
Oe Tomoyuki 東北大学, 薬学研究科, 教授 (10203712)
|
Co-Investigator(Kenkyū-buntansha) |
李 宣和 東北大学, 薬学研究科, 准教授 (60519776)
幡川 祐資 東北大学, 薬学研究科, 助教 (30878351)
|
Project Period (FY) |
2021-07-09 – 2023-03-31
|
Keywords | エクスポゾーム / ケラチン |
Outline of Final Research Achievements |
Epidermal proteins, keratins, were used as recording media to estimate exposure of external stimuli (UV, environmental chemicals, etc.). As a result, analytical method to screen chemical modifications on keratins was developed. Furthermore, the variation due to UV irradiation and chemical exposure were confirmed. On the other hand, concentrations of exogenous small molecules retained on skin were too low to be detected under co-existence of the large amount of endogenous lipids. Therefore, derivatization with 4-bromophenylhydrazine followed by mass spectrometric screening using characteristic isotope patterns was developed for targeting analysis of volatile aldehydes. Furthermore, a nucleophilic peptide GHK present in the epidermis was found to be a possible trapping reagent for these electrophilic chemicals. Those detection systems developed here are expected to help for exhaustive exposome in future.
|
Free Research Field |
臨床分析化学
|
Academic Significance and Societal Importance of the Research Achievements |
本研究は、表皮ケラチンの「多様な化学修飾を受ける」、「疎水性低分子を保持できる」、「短周期で置き換わる」、「非侵襲的に採取できる」特徴を生かし、外部刺激の暴露の記録媒体としての利用を試みたものである。今回の成果である「ケラチンの化学修飾解析法の構築」と「表皮上の低分子化合物の検出系開発」は、環境中の化学物質の暴露量を、環境濃度のみならず、個々人の暴露状況(暴露時間・濃度)などを評価できる可能性を示したものであり、『エクスポゾーム』の方法論として意義がある。
|