2023 Fiscal Year Final Research Report

"Development of an Artificial Human Vascular Bed for Vascularization and Sustained Culture of Three-Dimensional Tissues"

Research Project

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Project/Area Number	21K19887
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 90:Biomedical engineering and related fields
Research Institution	Hiroshima University (2023) The University of Tokyo (2021-2022)
Principal Investigator	Miura Shigenori 広島大学, 医系科学研究科(歯), 准教授 (70511244)
Co-Investigator(Kenkyū-buntansha)	聶銘昊東京大学, 大学院情報理工学系研究科, 講師 (40816485)
Project Period (FY)	2021-07-09 – 2024-03-31
Keywords	血管ベッド / BioMEMS / 灌流培養 / 血管導入 / 長期培養 / オルガノイド / 管腔形成 / マイクロ流路
Outline of Final Research Achievements	This study aims to develop an artificial human vascular bed device capable of long-term culture through vascular integration and perfusion into three-dimensional cultured tissues. We designed and fabricated an original vascular bed device in which a microchannel and a circular open chamber were arranged adjacently via a micropost structure. By introducing and culturing human vascular endothelial cells in composite hydrogel into the device, we successfully constructed a 6 mm diameter artificial human vascular bed with a luminal structure. Furthermore, we achieved partial perfusion of the vascular bed by optimizing factors such as cellular composition and the method of medium introduction, though further investigation is needed regarding the method of vascular integration into the tissue.
Free Research Field	組織工学
Academic Significance and Societal Importance of the Research Achievements	近年、組織工学やオルガノイドを用いた生体外組織モデルの研究が注目を集めているが、より大型の組織または発生後期の臓器を構築するためには、組織への血管導入および灌流培養の実現が喫緊の課題である。本研究で開発を進めてきた人工ヒト血管床デバイスは、マイクロ流路に連結された人工ヒト血管床をミリメートルサイズのオープンチャンバー内に構築したもので、マイクロ流路に送液することで血管床の灌流培養が可能である。今後、チャンバー内に設置した３次元組織と人工血管網との効果的な連結を実現できれば、生体外における“組織/臓器生育デバイス”としての応用が期待される。