2022 Fiscal Year Final Research Report
Induction of DNA dispersion by mechanical stimulation of the nucleus and its possible application to cell function control
| Project/Area Number |
21K19902
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
前田 英次郎 名古屋大学, 工学研究科, 准教授 (20581614)
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| Project Period (FY) |
2021-07-09 – 2023-03-31
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| Keywords | メカノバイオロジー / 細胞核 / クロマチン / DNA / 力学刺激 |
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| Outline of Final Research Achievements |
Osteoblast-like cells MC3T3-E1 were passed through a microchannel having a narrow portion of 10 um in width and 100 um in length to compress the cell nuclei. The number of intranuclear DNA aggregates decreased after passage through the channel, but the total volume did not change, suggesting the possibility that the aggregates fused together. In addition, the cell proliferation rate decreased after passage through the channel. When dynamic compression with an amplitude of 1 um and a frequency of 800 Hz was applied to the S-phase nuclei for 5 min, the number of aggregates decreased and the projected area did not change significantly. In the non-S phase, the number of aggregates and the projected area increased significantly. The DNA become loosened due to replication in the S phase. This may disassemble the aggregates easily. In addition, the loosened DNA may push the nuclear membrane from the inside and it may make the nucleus less likely to collapse.
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| Free Research Field |
バイオメカニクス
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| Academic Significance and Societal Importance of the Research Achievements |
細胞核を圧縮することでクロマチン凝集体の個数や総体積が変化することは判っていたが，細胞周期の影響があることが確認できた点，また，その影響がＳ期と非Ｓ期のクロマチンの凝集状態の違いから説明できそうであることが判った点が第１の成果である．またこれまでは凝集体の個数や体積の変化の理解に留まっていたが，マイクロ流路を通すことにより，増殖能にも変化が現れることが判った点が第２の成果と言える．
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