2023 Fiscal Year Final Research Report
Development of innovative Muse cell-based therapies for idiopathic osteonecrosis of the femoral head
| Project/Area Number |
21K19924
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
Yamada Hiroshi 和歌山県立医科大学, 医学部, 教授 (70275361)
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| Co-Investigator(Kenkyū-buntansha) |
原田 悌志 和歌山県立医科大学, 医学部, 博士研究員 (20838320)
南方 邦彦 和歌山県立医科大学, 医学部, 準客員研究員 (50838307)
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| Project Period (FY) |
2021-07-09 – 2024-03-31
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| Keywords | 特発性大腿骨頭壊死 / Muse細胞 / 動物モデル / ステロイド / 治療効果 |
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| Outline of Final Research Achievements |
The therapeutic effect of Muse cells on an animal model (rat) of idiopathic osteonecrosis of the femoral head was investigated. After the model was created, a large dose of steroids was administered. Two weeks after administration, the femoral head was harvested and histopathologically assessed for the development of osteonecrosis. Pathological findings suggestive of necrosis within the diaphyseal structures were observed in 12 heads of 11 out of 30 rats used. The animal model created was injected with 300,000 Muse cells intravenously. In some cases, the progression of necrosis of the femoral head was suppressed, but this was not significantly different from the group that received 300,000 non-Muse cells. In the present experimental system, it was difficult to supply a stable model due to problems with the animals used, so comparative studies with a sufficient number of animals were not possible, and the therapeutic effect of Muse cells could not be objectively proven.
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| Free Research Field |
整形外科学
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| Academic Significance and Societal Importance of the Research Achievements |
今回採用した動物モデルは、多くが大腿骨頭の骨化障害や骨化遅延を伴っており、骨梁構造が十分に確認できなかったため、骨頭壊死の有無を判定することは困難であった。このため、品種、生産業者の変更を行ったが、改善されなかった。しかしながら、Muse細胞がステロイド誘発性大腿骨頭壊死に対し、抗炎症作用、抗線維化作用により壊死の進行を抑制するとともに壊死部の再生に寄与している可能性は大いにあると考えている。今後、動物モデルの変更又は骨化障害・骨化遅延を生じない品種を検索し、本実験系を継続していく予定である。
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