Functinoal analysis of MGST, Prunus-specific pollen part self incompatibility factor

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20581
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0602:Agricultural and environmental biology and related fields
• Research Institution: Kagawa University
• Principal Investigator: Ono Kentaro 香川大学, 農学部, 助教 (10910896)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: バラ科サクラ属果樹 / 自家不和合性 / タンパク質機能解析 / グルタチオン-S-トランスフェラーゼ

Outline of Final Research Achievements

Molecular function of MGST was analyzed in this study. MGST is the protein which functions in the self-incompatibility reaction of Prunus, but not in the other species. The protein-protein interaction analysis of MGST and S-RNase showed that these two proteins interact in a limited condition. Predicted conformation of MGST was similar to that of the proteins which catalyze the oxidoreduction of the disulfide bond of their target molecules. On the other hand, MGST did not exhibit glutathione conjugation activity, disulfide reduction activity, or the ability to regulate the activity of S-RNase.

Free Research Field

果樹園芸学

Academic Significance and Societal Importance of the Research Achievements

自家不和合性を示す植物は自家受粉により果実や種子が得られないため，果実生産現場や育種現場，遺伝解析研究においては自家不和合性を自由に人為的に制御できることが理想である．人為制御のためにはメカニズムの解明が必要であるが，バラ科サクラ属植物の自家不和合性は他種と異なる独自のメカニズムを有し，そのメカニズム解明は学術的にも興味を引くものである．本研究では，サクラ属に特異的な自家不和合性因子MGSTの機能解析を行い，サクラ属に特異的なメカニズムの解明に役立つ情報を得た．