Functional analysis of pathogen effector recognition site of rice blast resistant protein

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20586
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0602:Agricultural and environmental biology and related fields
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Miyaji Naomi 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究員 (60908383)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: 抵抗性遺伝子 / Integrated Domain / エフェクター / イネ / いもち病

Outline of Final Research Achievements

This work aims to elucidate the function of the integrated domain of the resistance protein. I focused on the relationship between the rice blast resistance protein and the rice blast effector. Analysis of the interaction between the resistance protein and the effector identified the region of the resistance protein expected to be important for effector recognition. I also investigated the effect of the effector on the rice immune response using gene expression analysis and reactive oxygen species (ROS) production measuring method, and found that the rice blast effector suppressed the rice immune response.

Free Research Field

遺伝育種科学

Academic Significance and Societal Importance of the Research Achievements

本研究により、エフェクターの認識には抵抗性タンパク質の複数の領域が重要である可能性が示唆された。また、本研究で着目したいもち病菌エフェクターがイネの免疫応答を抑制する機能を持つことが示された。イネといもち病菌の相互作用メカニズムは未解明な部分が多い。本研究で得られた成果は、宿主と病原菌の相互作用メカニズムを今後解明する上で重要な知見となる。