Development of a new functional analysis using mutational profiling that reveals RNA-protein interaction

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20638
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0701:Biology at molecular to cellular levels, and related fields
• Research Institution: Ehime University
• Principal Investigator: Yamagami Ryota 愛媛大学, 理工学研究科(工学系), 助教 (70767227)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: RNA / tRNA / tRNA修飾 / tRNA修飾酵素 / 網羅解析 / 変異プロファイリング法

Outline of Final Research Achievements

The objective of this project is to develop a new high-throughput technique for conducting functional analyses of RNA modification enzymes. The findings have been reported as follows: (1) We have developed tRNA-MaP, a technique that selectively detects substrate tRNAs from a mixture of substrate and non-substrate tRNAs. (2) By using tRNA-MaP, we were able to reveal the substrate tRNA recognition mechanism of tRNA m1A22 methyltransferase (TrmK) from Geobacillus stearothermophilus, a thermophilic bacteria. (3) In combination with protein orthology analysis, tRNA-MaP was used to predict the tRNA modification enzyme genes encoded in the G. stearothermophilus genome.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

本研究は、RNAとRNA修飾酵素の相互作用機構とその機能の理解を深めることを目的としています。本研究によって開発されたtRNA-MaP法は、本目的の達成を加速させるものであり、幅広い分野での応用が期待できます。
This project aims to enhance our understanding of RNA modification enzymes and their functions, and the developed tRNA-MaP technique has potential implications for further studies in this field.