2022 Fiscal Year Final Research Report
Understanding the mechanism of establishment of apical-basal axis~Spatio-temporal coordination of cell polarization and division timing~
Project/Area Number |
21K20650
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0702:Biology at cellular to organismal levels, and related fields
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Research Institution | Tohoku University |
Principal Investigator |
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Project Period (FY) |
2021-08-30 – 2023-03-31
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Keywords | 胚発生 / カルシウム / ライブイメージング / 細胞内動態 / シロイヌナズナ |
Outline of Final Research Achievements |
Body axis formation is the first developmental event required for a unicellular zygote to develop into a multicellular organism. In most flowering plants, apical-basal axis is established by the asymmetrical zygote division. To achieve asymmetric division, the finishing of polarization and the timing of division must be coordinated, but the detailed mechanism is unknown. In this study, we performed live-cell imaging of Arabidopsis zygote with some markers to analyze the transition of polarization, Ca wave dynamics, and cell cycle progression. Also, we clarified the sequence and relationships among them. As a result, we found that the growth rate of zygote is not constant, but transfer rapid growth stage just before division, as well as the change of Ca wave dynamics and cell cycle progression that occur before and after this phase. In the future, we expect to elucidate the mechanism of spatio-temporal coordination by exploring the control mechanism of this rapid growth stage.
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Free Research Field |
植物発生
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Academic Significance and Societal Importance of the Research Achievements |
これまで植物の受精卵では、細胞伸長や核の移動という極性化が非対称分裂に必須だと判明している一方で、分裂タイミングを決める時間的制御の理解は進んでいなかった。本研究では、細胞周期の解析から、発生最初期の時間的制御の一端を解明することに世界で初めて成功した。細胞の極性化や非対称分裂は、植物の発生全般で見られる重要な現象であるため、本研究の知見は植物の発生の原理への理解につながる。また、受精卵は個体発生の原点であり、正常に極性化や非対称分裂することは、正常な種子・個体形成にも必要である。ゆえに本研究は、農業や育種といった応用研究への基盤として重要である。
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