Contributuion of novel myeloid macrophage-like cells to pathology of bone

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20772
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0803:Pathology, infection/immunology, and related fields
• Research Institution: Kyoto University
• Principal Investigator: Fujii Takayuki 京都大学, 医学研究科, 特定助教 (30911557)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: 関節リウマチ / マクロファージ

Outline of Final Research Achievements

We performed flow cytometry and identified two distinct macrophage populations in the synovium and bone in the arthritic joint using SKG mice, as we previously reported in another mouse model. We sorted macrophage populations from bone and synovium separately and conducted RNA sequencing, and found that those macrophage subpopulations had distinct transcriptomic landscapes across cell types and tissues. Even though they share the same surface marker expressions, it was estimated that those macrophage subpopulations had different pathological functions in the pathology of joint arthritis.

Free Research Field

医学

Academic Significance and Societal Importance of the Research Achievements

関節リウマチ等の病態に関連する骨の破壊は、日常生活の質、社会生活における活動度や、時に生命予後に関連する。マクロファージは炎症を介して、骨破壊に関連することが知られている。関節リウマチモデルマウスの関節滑膜と骨のマクロファージを分析したところ、関節滑膜と骨のマクロファージは遺伝子発現パターンに大きな違いがあることが分かり、関節炎や骨破壊といった種々の障害に個別のアプローチが可能になる可能性がある。