2022 Fiscal Year Final Research Report
Development of diagnostic system and treatment for biliary cancer with lymph node metastasis using organoids and genome analysis
| Project/Area Number |
21K20850
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0901:Oncology and related fields
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| Research Institution | Tohoku University (2022)
Tokyo Women's Medical University (2021) |
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Principal Investigator |
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| Project Period (FY) |
2021-08-30 – 2023-03-31
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| Keywords | 胆道癌 / オルガノイド / ゲノム解析 / exome / transcriptome / 個別化医療 |
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| Outline of Final Research Achievements |
I purchased specific reagents, preservation solutions, nucleic acid extraction kits, etc. necessary for patient-derived organoid culture, succeeded in culturing several types of biliary cancer organoids, and created cell library. I compared the results of immunostaining and exome sequence of organoids with that of primary tumors, and confirmed that the organoid inherited specific mutations and expressions from primary tumors. Organoids that were able to be observed their growth/development morphology by time-lapse observation with a confocal microscope. In addition to biliary cancer, I also succeeded in culturing organoids derived from intraductal papillary mucinous neoplasm of pancreas, and were able to be observed the specific growth morphologies.
Furthermore, we could confirm the efficacy of specific therapeutic agents for driver gene mutations extracted from the results of exome and transcriptome sequences in organoids.
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| Free Research Field |
肝胆膵外科
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| Academic Significance and Societal Importance of the Research Achievements |
胆道癌の患者由来オルガノイド(三次元培養細胞)の培養に成功し、癌モデルとしての活用、研究を行っている。予後不良で治療法の少ない胆道癌の増殖/進展形態を生体内に近い形で観察し、薬剤感受性試験などを行う事ができている。
過去の保存切除検体のゲノム解析結果と臨床的特徴や予後をひもづけて、オルガノイドをin vitroモデルとして検証することで、新規抗腫瘍薬や悪性度予測ツールなどの開発につなげることができる。
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