2022 Fiscal Year Final Research Report
Development of rapidly constructible bone regeneration materials to treat large bone defect
Project/Area Number |
21K20978
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0906:Surgery related to the biological and sensory functions and related fields
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Research Institution | Kitasato University |
Principal Investigator |
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Project Period (FY) |
2021-08-30 – 2023-03-31
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Keywords | 局所硬化ゲル / 多孔質材料 / 骨欠損治療 |
Outline of Final Research Achievements |
We examined the effectiveness of in situ-formed gels (IFGs) consisting of hyaluronan (HyA)-tyramine (TA), horseradish peroxidase and hydrogen peroxide enhance the bone formation ability of hydroxyapatite (Hap)/BMP-2 composites for facilitating osteogenesis in refractory fracture model mice. After establishing the refractory fracture model, animals were either treated at the site of fracture with IFGs-HyA with Hap harboring BMP-2 (IFGs-HyA/Hap/BMP-2). We determined the extent of bone formation at the fracture site according to findings on micro-computed tomography and histological experiments. Animals treated with IFGs-HyA/Hap/BMP-2 demonstrated significantly greater bone volume, bone mineral content and bone union than those treated with vehicle or IFG-HyA/Hap alone. IFGs-HyA/Hap/BMP-2 could be an effective treatment option for refractory fractures.
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Free Research Field |
整形外科学
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Academic Significance and Societal Importance of the Research Achievements |
局所硬化ゲルの多孔質材料への成長因子導入法を確立し、その有用性を示した。本方法は骨欠損治療のみならず、再生医療全般に応用できるものと考えられる。
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