Development of rapidly constructible bone regeneration materials to treat large bone defect

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20978
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0906:Surgery related to the biological and sensory functions and related fields
• Research Institution: Kitasato University
• Principal Investigator: Shoji Shintaro 北里大学, 医学部, 助教 (60728202)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: 局所硬化ゲル / 多孔質材料 / 骨欠損治療

Outline of Final Research Achievements

We examined the effectiveness of in situ-formed gels (IFGs) consisting of hyaluronan (HyA)-tyramine (TA), horseradish peroxidase and hydrogen peroxide enhance the bone formation ability of hydroxyapatite (Hap)/BMP-2 composites for facilitating osteogenesis in refractory fracture model mice. After establishing the refractory fracture model, animals were either treated at the site of fracture with　IFGs-HyA with Hap harboring BMP-2 (IFGs-HyA/Hap/BMP-2). We determined the extent of bone formation at the fracture site according to findings on micro-computed tomography and histological experiments.　Animals treated with IFGs-HyA/Hap/BMP-2 demonstrated significantly greater bone volume, bone mineral content and bone union than those treated with vehicle or IFG-HyA/Hap alone.　IFGs-HyA/Hap/BMP-2 could be an effective treatment option for refractory fractures.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

局所硬化ゲルの多孔質材料への成長因子導入法を確立し、その有用性を示した。本方法は骨欠損治療のみならず、再生医療全般に応用できるものと考えられる。