Controlled release of protein factors for the treatment of cleft palate by tissue engineering

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K21014
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Hiroshima University
• Principal Investigator: Nakano Ayana 広島大学, 医系科学研究科(歯), 研究員 (60911568)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: 顎裂部閉塞治療 / 組織工学 / 細胞成長因子 / キメラタンパク質 / 高次構造予測 / コラーゲン / 間葉系幹細胞 / ケモカイン

Outline of Final Research Achievements

With the aim of establishing an effective tissue engineering method for the treatment of cleft palate, an attempt was made to design collagen-based scaffolds incorporating stromal cell-derived factor-1alpha, bone morphogenetic factor-2, and vascular endothelial growth factor. For this purpose, collagen-binding domain (CBD) was fused to these factors. In this study, special attention was paid to the effect of CBD on the structure and function of fused factors. To evaluate the higher order structure, an AI-based computer program was used and the results were experimentally validated. As a result, basic knowledge could be obtained on the structures and functions of these chimeric proteins. Furthermore, it was suggested that the program used in this study is useful for predicting higher-order structure of chimeric proteins.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、骨組織再生法の発展に寄与するものである。それによって、従来の骨移植法に代わる新たな顎裂部閉塞治療法が確立されれば、矯正歯科分野における恩恵は計り知れない。また、骨再生は顎裂部閉鎖術のみならず、医療の広い分野において待望される手法であるため、本研究の成果は、歯学のみならず、医学の進歩にも大きな貢献を果たすと考えられる。