2021 Fiscal Year Research-status Report
The combined application of mineral trioxide aggregate containing phosphorylated pullulan and colloidal platinum nanoparticles to control inflammation and facilitate dental pulp regeneration
| Project/Area Number |
21K21028
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| Research Institution | Hokkaido University |
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Principal Investigator |
ISLAM RAFIQUL 北海道大学, 歯学研究院, 博士研究員 (00906414)
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| Project Period (FY) |
2021-08-30 – 2023-03-31
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| Keywords | Direct pulp capping / Platinum Nanoparticles / Phosphorylated pullulan / MTA / Dental pulp |
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| Outline of Annual Research Achievements |
We evaluated the healing response of three different Colloidal platinum nanoparticles (CPN) materials in rodent dental pulp at day 1, 3 and 7 after direct capping. The teeth pulps were intentionally exposed and randomly divided into three groups according to the application of the CPN materials: group 1- PAA-PT (platinum, poly acrylic acid, water), group 2- C-PT (platinum, sodium citric acid, water) and group 3- C-CYD (platinum, sodium citric acid, γ-cyclodextrin, water).Additional two groups were included as positive control (Mineral Trioxide Aggregate-MTA) and negative control (Super Bond-SB). At day 1, no or mild inflammatory responses were observed in all three groups of CPN, which were significantly different to the MTA and SB groups (P<0.05), except C-PT group. At day 3, numerous blood vessels with no or mild inflammation were observed in the C- CYD group, indicating rapid angiogenesis, which was significantly different from MTA and SB groups (P<0.05), whereas PAA-PT and C-PT groups showed mild to moderate inflammatory responses. At day 7, no or mild inflammation was observed in the C-CYD group which was significantly different from PAA-PT, C-PT and SB (P<0.05), whereas mild or moderate inflammatory responses were observed in all other groups.
Our observations have been accepted for the conference presentation in the 100th General Session of the IADR/APR, 2022 (Abstract ID: 3723202; Title: Colloidal platinum nanoparticles enhance the healing potential of dental pulp).
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
Last year, we evaluated the healing responses of the colloidal platinum nanoparticles.
We are currently preparing the long-term samples for the three different colloidal platinum nanoparticles. We are also evaluating three different types of antibodies (CD31, Nestin, and DMP1) to confirm the vasculogenesis, odontoblast cells, and mineralized tissue formation. On the other hand, mineral trioxide aggregate containing phosphorylated pullulan integrated with colloidal platinum nanoparticles will be evaluated in the present fiscal year.
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| Strategy for Future Research Activity |
Long-term histological preparation: by the end of July 2022, Immunohistochemical preparation: by the end of August 2022, Data acquisition: by the end of September 2022, Data analysis: by the end of October 2022, Preparation of a draft for the manuscript: by the end of January 2023, Manuscript submission for publication: by the end of March 2023
In our present study evaluation, the healing responses of colloidal platinum nanoparticles on dental pulp were very satisfactory. Therefore, mineral trioxide aggregate combined with colloidal platinum nanoparticles could be the material of choice for direct pulp capping, which will be our next future research work.
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