2023 Fiscal Year Annual Research Report
roteomic, metagenomic and genome-wide analyses of oral cavity of ovariectomized rats with psychological stress
| Project/Area Number |
21K21094
|
|---|
| Research Institution | Health Sciences University of Hokkaido |
|---|
Principal Investigator |
PAUDEL Durga 北海道医療大学, 先端研究推進センター, 助教 (20910590)
|
|---|
| Project Period (FY) |
2021-08-30 – 2024-03-31
|
| Keywords | psychological stress / salivary gland / trancriptome / RNA-seq |
|---|
| Outline of Annual Research Achievements |
The research plan was to identify proteomic, metagenomic, and genome-wide alteration in the oral cavity under psychological stress and post-menopausal condition using an animal model. Various experiments were conducted to meet the research goal. One of the most important findings was from the genome-wide analysis of the salivary glands under psychological stress condition. The study identified some important genes that were altered in the salivary glands of rats due to psychological stress conditions. The findings have been published in an international journal (J Oral Pathol Med. 2024;53(2):150-158. doi:10.1111/jop.13512). The results showed differentially expressed genes between the stress group and the control. Among the differentially expressed genes, FosB, the most downregulated gene may hint that the submandibular gland could be involved in stress response. The downregulation of FosB in the submandibular gland may be a novel mechanism in psychological stress. The genesets for gene ontology cellular component, molecular function, and KEGG showed that pathways related to ribosome biosynthesis and function were downregulated in the stress group compared to the control.The findings of the present study are important in understanding the effect of psychological stress on the oral cavity. The altered gene expression and molecular pathways in the submandibular gland under psychological stress may affect salivary flow, alter the salivary proteins, or change the physical properties of saliva.
|
