2023 Fiscal Year Final Research Report
Generation of functional salivary glands using conditional blastocyst complementation and large animals
Project/Area Number |
21KK0290
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Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 57040:Regenerative dentistry and dental engineering-related
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Research Institution | Showa University |
Principal Investigator |
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Project Period (FY) |
2022 – 2023
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Keywords | 唾液腺 / 胚盤胞補完法 / 多能性幹細胞 |
Outline of Final Research Achievements |
Ablation of Fgfr2 within the Foxa2 lineage in mice leads to salivary gland agenesis. We reversed this phenotype by injecting donor pluripotent stem cells into the mouse blastocysts, resulting in mice that survived to adulthood with salivary glands of normal size, comparable to those of their littermate controls. These findings demonstrate that CBC-based salivary gland regeneration serves as a foundational experimental approach for future advanced cell-based therapies.
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Free Research Field |
再生医療
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Academic Significance and Societal Importance of the Research Achievements |
口腔乾燥症(ドライマウス)の潜在的患者数は800万人ともいわれており、齲蝕、口腔感染症および摂食嚥下障害などの罹患率の上昇がみられる。現在根治療法が存在しないため唾液腺再生医療の開発が切望されている。 本研究では、胚盤胞補完法を用いてES細胞およびiPS細胞からマウス体内で野生型と同等のサイズおよび組織構造を伴う唾液腺の作出に成功した。これらの成果は、将来的な大動物を用いた胚盤胞補完法によりヒトサイズの唾液腺を作出するための基盤技術となり得る。
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