2014 Fiscal Year Final Research Report
Structural and functional study of membrane proteins based on electron crystallography
| Project/Area Number |
22227004
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University (2012-2014)
Kyoto University (2010-2011) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHSHIMA Atsunori 名古屋大学, 大学院創薬科学研究科/細胞生理学研究センター, 准教授 (80456847)
ABE Kazuhiro 名古屋大学, 大学院創薬科学研究科/細胞生理学研究センター, 助教 (60596188)
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Keywords | 電子線結晶学 / 膜タンパク質 / 水チャネル / イオンチャネル / ギャップ結合チャネル / クローディン / アセチルコリン受容体 / H+,K+-ATPase |
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| Outline of Final Research Achievements |
In this project, we studied structure and function of water channels, ion channels, gap junction channels, acetylcholine receptor, claudins and H+,K+-ATPase, because these membrane proteins are important components in biological systems. We could obtain significant results in these research subjects by mainly electron and X-ray crystallography.
For example, we intensively studied adhennels, channels with adhesive function. Claudin is an adhennel family protein and a key molecule in tight junctions. We analyzed structure of claudin-15 and proposed a paracellular channel model to explain channel function through epithelial cell sheets. We also analyzed the complex structure of claudin-19 and the C-terminal domain of Clostridium perfringens enterotoxin, which causes disintegration of tight junctions. These results are of great interest for both basic biology and the rational design of therapeutic agents that can potentially be used to deliver drugs across tissue barriers.
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| Free Research Field |
構造生理学
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