2012 Fiscal Year Final Research Report
Transcranial optical imaging of neural activity in deep brain structurtes in mice
| Project/Area Number |
22240044
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fusional basic brain science
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HISHIDA Ryuichi 新潟大学, 脳研究所, 准教授 (90313551)
TOHMI Manabu 新潟大学, 脳研究所, 助教 (10401770)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 脳機能イメージング / マクロ共焦点顕微鏡 / 近赤外光 / 活動依存的血流変化 |
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| Research Abstract |
We developed a new optical method for imaging neural activity in deep brain structures in mice. We used a macroconfocal microscope in a near-infrared range. Generally,a confocal microscope uses only a part of light passing through a tiny pinhole. Therefore,the diameter of a pinhole was expanded, and sensitivity of our macroconfocal microscopewas strengthened. We labeled red blood cells (RBC) with NIR815, a near-infraredfluorescent probe, and applied the fluorescent RBCs to mice. In these mice,activity-dependent hemodynamic responses elicited by visual stimuli were successfullyimaged in the lateral geniculate body, which located approximately 2.5 mm deep from thecortical surface.
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Research Products
(21 results)
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[Journal Article] Spatiotemporal dynamics ofepileptiform propagations: imaging ofhuman brain slices2011
Author(s)
Kitaura H, Hiraishi T, Murakami H, Masuda H, Fukuda M, Oishi M,Ryufuku M, Fu YJ, Takahashi H,Kameyama S, Fujii Y, Shibuki K,Kakita A
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Journal Title
Neuroimage
Volume: 58
Pages: 50-59
Peer Reviewed
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