Establishment of the methodology for design and growth of implantable pancreatic beta cell or liver cell tissues in vitro

2014 Fiscal Year Final Research Report

• Project/Area Number: 22246101
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: The University of Tokyo
• Principal Investigator: SAKAI Yasuyuki 東京大学, 生産技術研究所, 教授 (00235128)
• Co-Investigator(Kenkyū-buntansha): SHIRAKASHI Ryo 東京大学, 生産技術研究所, 教授 (80292754) ; ITOH Taichi 東京大学, 大学院医学系研究科, 准教授 (50447421)
• Co-Investigator(Renkei-kenkyūsha): NIINO Toshiki 東京大学, 生産技術研究所, 教授 (70291929)
• Research Collaborator: YUAN Pang ; YAO-TONG Lai ; STEPHANIE Liana Utami Sutoko
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 医用化学工学 / マルチスケール血管網配備 / 膵島 / 肝 / 三次元造形 / 人工赤血球

Outline of Final Research Achievements

Toward engineering implantable pancreatic beta cell or liver cell tissues, an integrative methodologies that fully utilizes the advantages of both tissue element assembly in a micro-cale and large 3D scaffold having a flow channel network in a macro-scale. Model scaffolds was fabricated by the 3D selective laser sintering (SLS) process. Good results of the perfusion culture of liver cell aggregates proved the high feasibility of the methodology. In addition, we succeeded in the preparation of hemoglobin-based artificial oxygen carrier showing lower cytotoxicity.

Free Research Field

臓器・生体システム工学