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2013 Fiscal Year Final Research Report

Evolutional strategy for the establishment of angiosperm-type chloroplast NDH

Research Project

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Project/Area Number 22247005
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

SHIKANAI TOSHIHARU  京都大学, 理学(系)研究科(研究院), 教授 (70273852)

Co-Investigator(Renkei-kenkyūsha) TSUGEKI Ryuuji  京都大学, 大学院理学研究科, 助教 (50303805)
NISHIMURA Yoshiki  京都大学, 大学院理学研究科, 助教 (70444099)
Project Period (FY) 2010-04-01 – 2013-03-31
Keywords葉緑体 / NDH / サイクリック電子伝達 / 光合成 / アセンブリ / 進化 / シロイヌナズナ / ゼニゴケ
Research Abstract

We discovered that a novel protein, NdhS is necessary for the high-affinity binding of chloroplast NDH with ferredoxin. Chloroplast NDH is not NAD(P)H dehydrogenase but antimycin A-resistant ferredoxin-dependent plastoquinone reductase (FQR). We also clarified the assembly process of subcomplex A of chloroplast NDH in Arabidopsis with the discovery of several assembly factors specifically required for this process. We further discovered that in Marchantia chloroplast NDH does not form the supercomplex with photosystem I and is involved in the redox homeostasis in chloroplasts at low light intensity.

  • Research Products

    (25 results)

All 2014 2013 2012 2011 2010

All Journal Article (12 results) Presentation (13 results)

  • [Journal Article] Central role of cyclic electron transport around photosystem I in the regulation of photosynthesis2014

    • Author(s)
      Shikanai T
    • Journal Title

      Curr. Opin. Biotech.

      Volume: 26 Pages: 25-30

    • DOI

      10.1016/j.copbio.2013.08.012

  • [Journal Article] In planta mutagenesis of Src homology 3 domain-like fold of NdhS, a ferredoxin-binding subunit of the chloroplast NADH dehydrogenase-like complex in Arabidopsis. A conserved Arg-193 plays a critical role in ferredoxin binding2013

    • Author(s)
      Yamamoto H. and Shikanai T
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Pages: 36328-36337

    • DOI

      10.1074/jbc.M113.511584

  • [Journal Article] Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts2013

    • Author(s)
      Taira Y., Okegawa Y., Sugimoto K., Abe M., Miyoshi H. and Shikanai T
    • Journal Title

      FEBS Open Bio.

      Volume: 3 Pages: 406-410

    • DOI

      10.1016/j.fob.2013.09.007

  • [Journal Article] A single amino acid alteration in PGR5 confers resistance to antimycin A in cyclic electron transport around PSI2013

    • Author(s)
      Sugimoto K., Okegawa Y., Tohri A., Long T.A., Sarah F.S., Hisabori T. and Shikanai T
    • Journal Title

      Plant Cell Physiol.

      Volume: 54 Pages: 1525-1534

    • DOI

      10.1093/pcp/pct098

  • [Journal Article] PGR5-dependent cyclic electron transport around PSI contributes to the redox homeostasis in chloroplasts rather than CO_2 fixation and biomass production in rice2012

    • Author(s)
      Nishikawa Y., Yamamoto H., Okegawa Y., Wada S., Sato N., Taira Y., Sugimoto K., Makino A. and Shikanai T
    • Journal Title

      Plant Cell Physiol.

      Volume: 53 Pages: 2117-2126

    • DOI

      10.1093/pcp/pcs153

  • [Journal Article] Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha2012

    • Author(s)
      Ueda M., Kuniyoshi T., Yamamoto Y., Sugimoto K., Ishizaki K., Kohchi T., Nishimura Y. and Shikanai T
    • Journal Title

      Plant J.

      Volume: 72 Pages: 683-693

    • DOI

      10.1111/j.1365-313X.2012.05115.x

  • [Journal Article] Multistep assembly of chloroplast NADH dehydrogenase-like subcomplex A requires several nucleus-encoded proteins, including CRR41 and CRR42, in Arabidopsis2012

    • Author(s)
      Peng L., Fukao Y., Fujiwara M. and Shikanai T
    • Journal Title

      Plant Cell

      Volume: 24 Pages: 202-214

    • DOI

      10.1105/tpc.111.090597

  • [Journal Article] Structure of the chloroplast NADH dehydrogenase-like complex : nomenclature for nuclear-encoded subunits2011

    • Author(s)
      Ifuku K., Endo T., Shikanai T. and Aro E.-M
    • Journal Title

      Plant Cell Physiol.

      Volume: 52 Pages: 1560-1568

    • DOI

      10.1093/pcp/pcr098

  • [Journal Article] An Src homology 3 domain-like fold protein forms a ferredoxin-binding site for the chloroplast NADH dehydrogenase-like complex in Arabidopsis2011

    • Author(s)
      Yamamoto H., Peng L., Fukao Y. and Shikanai T
    • Journal Title

      Plant Cell

      Volume: 23 Pages: 1480-1493

    • DOI

      10.1105/tpc.110.080291

  • [Journal Article] A chaperonin subunit with unique structures is essential for folding of a specific substrate2011

    • Author(s)
      Peng L., Fukao Y., Myouga F., Motohashi R., Shinozaki K. and Shikanai T
    • Journal Title

      PLoS Biol.

      Volume: 9 Pages: e1001040

    • DOI

      10.1371/journal.pbio.1001040

  • [Journal Article] Supercomplex formation with photosystem I is required for the stabilization of the chloroplast NADH dehydrogenase-like complex in Arabidopsis2011

    • Author(s)
      Peng L. and Shikanai
    • Journal Title

      Plant Physiol.

      Volume: 155 Pages: 1629-1639

    • DOI

      10.1104/pp.110.171264

  • [Journal Article] Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase complex2011

    • Author(s)
      Peng L., Yamamoto H. and Shikanai T
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1807 Pages: 945-953

    • DOI

      10.1016/j.bbabio.2010.10.015

  • [Presentation] Machinery and function of PSI cyclic electron transport2014

    • Author(s)
      Shikanai T
    • Organizer
      2nd Kyoto-Bristol Symposium
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-01-10
  • [Presentation] Regulation of photosynthetic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      NC-CARP•CREST/さきがけ国際シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2013-11-08
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      PhD Summer School : New Frontiers in Photosynthesis
    • Place of Presentation
      San Michele all'Adige, Italy
    • Year and Date
      2013-07-30
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      24th International Conference on Arabidopsis Research
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2013-06-25
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      3^<rd> International Symposium on Chloroplast Genomics and Genetic Engineering
    • Place of Presentation
      New Brunswick, USA
    • Year and Date
      2013-05-11
  • [Presentation] Cyclic electron flow : new structural components and regulation2012

    • Author(s)
      Shikanai T
    • Organizer
      IPMB 2012
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2012-10-23
  • [Presentation] Assembly of the chloroplast NDH complex2012

    • Author(s)
      Shikanai T
    • Organizer
      The 11th Nordic Photosynthesis Congress
    • Place of Presentation
      Naantali, Finland
    • Year and Date
      2012-09-12
  • [Presentation] Chloroplast NADH dehydrogenase- like complex : function and assembly2012

    • Author(s)
      Shikanai T
    • Organizer
      Gordon Research Conference, Mitochondria & Chloroplasts
    • Place of Presentation
      Smithfield, USA
    • Year and Date
      2012-08-01
  • [Presentation] Structure and assembly of the chloroplast NDH complex2012

    • Author(s)
      Shikanai T
    • Organizer
      NU Plant Science Symposium 2012
    • Place of Presentation
      Jinju, Korea
    • Year and Date
      2012-05-11
  • [Presentation] Regulation of photosynthetic electron transport by PSI cyclic electron transport2011

    • Author(s)
      Shikanai T
    • Organizer
      The 15^<th> International Congress of Photosynthesis
    • Place of Presentation
      Beijing, China
    • Year and Date
      20110822-27
  • [Presentation] Machinery and assembly of PSI cyclic electron transport2011

    • Author(s)
      Shikanai T
    • Organizer
      Gordon Research Conference, Photosynthesis
    • Place of Presentation
      Davidson, USA
    • Year and Date
      20110612-17
  • [Presentation] Machinery and assembly of the chloroplast NDH complex2011

    • Author(s)
      Shikanai T
    • Organizer
      witzerland-Japan Workshop
    • Place of Presentation
      Ollon, Swiss
    • Year and Date
      20110110-14
  • [Presentation] Structure and function of machinery of photosystem I cyclic electron transport2010

    • Author(s)
      Shikanai T
    • Organizer
      APRU Research Symposium
    • Place of Presentation
      Kyoto
    • Year and Date
      20101124-26

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Published: 2015-07-16  

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