2014 Fiscal Year Final Research Report
FTIR spectroscopy of non-photoreceptive membrane proteins
Project/Area Number |
22247024
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Nagoya Institute of Technology |
Principal Investigator |
KANDORI Hideki 名古屋工業大学, 工学(系)研究科(研究院), 教授 (70202033)
|
Co-Investigator(Kenkyū-buntansha) |
古谷 祐詞 分子科学研究所 (80432285)
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Project Period (FY) |
2010-04-01 – 2015-03-31
|
Keywords | 赤外分光 / 膜タンパク質 / イオン / チャネル / レセプター |
Outline of Final Research Achievements |
In general, vibrational spectroscopy has never been recognized as one of structural biology tools, but we have shown that light-induced difference FTIR spectroscopy is a powerful tool for the analysis of structural changes in photoreceptive proteins for function. This project aims at establishing difference FTIR spectroscopy as a tool of similar analysis for non-photoreceptive membrane proteins. We optimized the experimental setup using attenuated total reflection (ATR) FTIR spectroscopy, which enabled to obtain difference FTIR spectra upon various stimuli in solution. We obtained structural information of ion-channels (potassium, sodium, proton and water channels), ion-pumps that specifically bind sodium and chloride ions, rotary motors such as V-type ATPase, GPCRs such as taste-receptor, and transporters such as POT. We reported more than 70 articles in Nature, Nature Commun., Nature Nanotech., JACS, Angew., PNAS, JPC Letters and JBC during the project period.
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Free Research Field |
生物物理学
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