Molecular Understanding of STOP1-regulating system in plant stress tolerance

2013 Fiscal Year Final Research Report

• Project/Area Number: 22248006
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Gifu University
• Principal Investigator: KOYAMA HIROYUKI 岐阜大学, 応用生物科学部, 教授 (90234921)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Yoshiharu 岐阜大学, 応用生物科学部, 教授 (50301784) ; IUCHI Satoshi 独立行政法人理化学研究所, 実験植物開発室, 研究員 (90312256) ; SUZUKI Yuji 東北大学, 農学研究科, 助教 (80374974) ; SAKURAI Nozomu (財)かずさDNA研究所, 植物ゲノムバイテク室, 研究員 (30392286)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: STOP1 / 植物免疫 / 酸性土壌耐性

Research Abstract

STOP1, a zinc finger protein, regulates transcription of Al and proton tolerance genes in Arabidopsis. We characcterized function of orthologous genes in various plant species, including woody plants, grass plants, and bryophyte. All plants conserved functional protein of STOP1-orthologues, which regulate expression of various acid and Alresistant genes. It indicated STOP1-regulating system is ubiquitus among wide range of land plant speceis.
Expression of ALMT1, encoding malate transporter, is strictly regulated by STOP1. Expression of the gene is activated by Al, but is also activated by bacterial elicitors. The former protects root tips from Al toxicity, but the latter recruites veneficial bacteria that induce plant immunity. It indecated that STOP1 and regulating genes are likely a module that has multiple function in stress registance of higher plants.

Research Products

• [Journal Article] Identification of a STOP1-like protein in Eucalyptus that regulates transcription of Al tolerance genes (2014)
  • Author(s): Sawaki Y., Kobayashi Y., Kihara-Doi T., Nishikubo N., Kawazu T., Kobyashi M., Kobayashi Y., Iuchi S., Koyama H.. & Sato S.
  • Journal Title: Plant Sci. Volume: 223 Pages: 8-15
  • Peer Reviewed
• [Journal Article] STOP2 activates transcription of several genes for Al- and low pH-tolerance that are regulated by STOP1 in Arabidopsis (2014)
  • Author(s): Kobayashi, Y., Ohyama, Y., Kobayashi, Y., Ito, H., Iuchi, S., Fujita, M., Zhao, C-R., Tanveer, T., Ganesan, M., Kobayashi, M. & Koyama H.
  • Journal Title: Mol Plant Volume: 7 Pages: 311-322
  • Peer Reviewed
• [Journal Article] Characterization of Al-responsive citrate excretion and citrate-transporting MATEs in Eucalyptus camaldulensis (2013)
  • Author(s): Sawaki,Y., Kihara-Doi, T., Kobayashi, Y., Nishikubo, N., Kawazu, T., Kobayashi, Y., Koyama, H. & Sato S.
  • Journal Title: Planta Volume: 237 Pages: 979–989
  • Peer Reviewed
• [Journal Article] Characterization of the complex regulation of AtALMT1 expression in response to phytohormones and other inducers (2013)
  • Author(s): Kobayashi, Y. Kobayashi, Y., Sugimoto, M., Lakshmanan, V., Iuchi, S., Kobayashi, M., Bais, H and Koyama, H.
  • Journal Title: Plant Physiol Volume: 162 Pages: 732-740
  • Peer Reviewed
• [Journal Article] Overexpression of AtALMT1 in the Arabidopsis thaliana ecotype Columbia results in enhanced Al-activated malate excretion and beneficial bacterium recruitment (2013)
  • Author(s): Kobayashi, Y., Lakshmanan, V., Kobayashi, Y., Asai, M., Iuchi, S., Kobayashi, M., Bais, H. P. & Koyama H.
  • Journal Title: Plant Signaling & Behavior Volume: 8(9) Pages: e-25565
  • Peer Reviewed
• [Journal Article] Characterization of AtSTOP1 orthologous genes in tobacco and other plant species (2013)
  • Author(s): Ohyama, Y., Ito, H., Kobayashi, Y., Ikka, T., Morita, A., Kobayashi, M., Imaizumi, R., Aoki, T., Komatsu, K., Sakata, Y., Iuchi, S. & Koyama, H.
  • Journal Title: Plant Physiol Volume: 162 Pages: 1937-1946
  • Peer Reviewed
• [Journal Article] Molecular and physiological analysis of Al3+ and H+ rhizotoxicities at moderately acidic conditions (2013)
  • Author(s): Kobayashi, Y., Kobayashi, Y., Watanabe, T., Shaff J. E., Ohta, H., Kochian, L. V., Wagatsuma, T., Kinraide, T. B. & Koyama H.
  • Journal Title: Plant Physiol Volume: 163 Pages: 180-192
  • Peer Reviewed
• [Journal Article] 他種由来STOP1 相同遺伝子を導入した相補組換え体シロイヌナズナの解析 (2012)
  • Author(s): 大山慶直,小林佑理子,井内聖,小山博之
  • Journal Title: 無菌生物 Volume: 42 Pages: 69-72
• [Journal Article] Analysis of environmental stress in plants with the aid of marker genes for H2O2 responses (2012)
  • Author(s): Hieno, A., Naznin, A.H. Sawaki, K., Koyama, H., Sakai, S., Ishino, H., Hyakumachi, M. & Yamamoto, Y.Y.
  • Journal Title: Methods Enzymol Volume: 527 Pages: 221-238
  • Peer Reviewed
• [Presentation] STOP1 Regulating System ; A Root Module for Adapting to Acid Soil Environment, and for Enhancing Plant Immunity (2013)
  • Author(s): Koyama H., Kobayashi Y., Bais H, Iuchi S.
  • Organizer: 第3回国際農業会議
  • Place of Presentation: 中国杭州
  • Year and Date: 20130900
• [Book] Rhizotoxic ions : 'Omics' approaches for studying abiotic stress tolerance in plants. In Omics and Plant Abiotic Stress Tolerance (2011)
  • Author(s): Zhao, C-Z., Yamamoto, Y.Y. & Koyama, H.
  • Total Pages: 128-132
• [Book] Heavy metal tolerance in Arabidopsis thaliana Advances in Botanical Research 60 (2011)
  • Author(s): Chaffai, R. & Koyama, H.
  • Total Pages: 4-20