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2012 Fiscal Year Final Research Report

Development of comparative metagenomics for soil microbiome analysis

Research Project

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Project/Area Number 22248038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionThe University of Tokyo

Principal Investigator

SENOO Keishi  東京大学, 大学院・農学生命科学研究科, 教授 (40206652)

Co-Investigator(Kenkyū-buntansha) OHTA Hiroyuki  茨城大学, 農学部, 教授 (80168947)
Co-Investigator(Renkei-kenkyūsha) HATTORI Masahira  東京大学, 新領域創成科学研究科, 教授 (70175537)
SHINANO Takuro  (独)農業・食品産業技術総合研究機構, 東北農業研究センター 農業放射線研究 センター, センター長 (20235542)
MINAMISAWA Kiwamu  東北大学, 大学院・生命科学研究科, 教授 (70167667)
OTSUKA Shigeto  東京大学, 大学院・農学生命科学研究科, 准教授 (10313074)
HASHIMOTO Tomoyoshi  (独)農業・食品産業技術総合研究機構, 中央農業総合研究センター, 上席研究員 (90343946)
YOH Muneoki  東京農工大学, 農学研究院, 教授 (50260526)
Project Period (FY) 2010 – 2012
Keywords生物環境 / 土壌メタゲノミクス / 土壌環境再生 / 窒素循環 / 水田土壌
Research Abstract

We tried to uncover the structure and function of microbial communities in soil ecosystem through soil metagenomics using the new DNA-sequencing technology. Novel microbial communities responsible for reduction reaction and nitrogen transformation in rice paddy soil were revealed. The resilience of soil microbial community to the volcanic eruption impact was revealed and the detailed picture of soil microbial genetic response to the long-term application of livestock liquid manure was obtained.

  • Research Products

    (30 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (25 results) Book (1 results)

  • [Journal Article] Analysis of early bacterial communities on volcanic deposits on the Island of Miyake (Miyake-jima)2012

    • Author(s)
      Reiko Fujimura, Yoshinori Sato, Tomoyasu Nishizawa, Kenji Nanba, Kenshiro Oshima, Masahira Hattori, Takashi Kamijo and Hiroyuki Ohta
    • Journal Title

      Japan: a 6-year study at a fixed site, Microbes and Environments

      Volume: 27 Pages: 19-29

    • DOI

      DOI:10.1264/jsme2.ME11207

    • Peer Reviewed
  • [Journal Article] Complete genome sequence of Leptospirillum ferrooxidans strain C2-3 isolated from fresh volcanic ash deposit on the island of Miyake2012

    • Author(s)
      Reiko Fujimura, Yoshinori Sato, Tomoyasu Nishizawa, Kenshiro Oshima, Seok-Won Kim, Masahira Hattori, Takashi Kamijo and Hiroyuki Ohta
    • Journal Title

      Japan, Journal of Bacteriology

      Volume: 194 Pages: 4122-4123

    • DOI

      DOI:10.1128/​JB.00696-12

    • Peer Reviewed
  • [Journal Article] 農業環境分野へのメタゲノム解析の展開2011

    • Author(s)
      伊藤英臣、石井聡、妹尾啓史
    • Journal Title

      日本水産学会誌

      Volume: 77巻 Pages: 254

    • DOI

      DOI:10.2331/suisan.77.254

    • Peer Reviewed
  • [Journal Article] 初成土壌環境における微生物-三宅島土壌 生態系再生のメカニズムを探る-2011

    • Author(s)
      藤村玲子、佐藤嘉則、難波謙二、 太田寛行
    • Journal Title

      日本生態学会誌

      Volume: 61巻 Pages: 211-218

    • Peer Reviewed
  • [Presentation] メタゲノム・メタトランスクリプトーム解析法を用いた水田土壌微生物群集の構造と機能の網羅的解明2012

    • Author(s)
      伊藤英臣、石井聡、白鳥豊、大島健志朗、大塚重人、 服部正平、 妹尾啓史
    • Organizer
      第28回日本微生物生態学会大会 第 4回日韓微生物生態シンポジウム
    • Place of Presentation
      豊橋技術科学大学、豊橋市
    • Year and Date
      2012-09-20
  • [Presentation] メタゲノム解析から明らかにされた三宅島初成土壌微生物生態系の遷移2012

    • Author(s)
      藤村玲子,金錫元,佐藤 嘉則,須田亙,西澤 智康,大島健志朗,服部正平,上條隆志,太田寛行
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学、豊橋市
    • Year and Date
      2012-09-20
  • [Presentation] スラリー長期連用圃場における土壌微生物生態系のメタゲノム解析2012

    • Author(s)
      石川美友紀,藤村玲子,新美洋,橋本知義,金錫元,須田亙,大島健志朗,服部正平,太田寛行
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学、豊橋市
    • Year and Date
      2012-09-20
  • [Presentation] メタゲノム 解析法を用いた水田土壌微生物群集の構造と機能の網羅的解明-メタゲノム解析から 見えた脱窒反応に関与する新たな微生物群集構造-2012

    • Author(s)
      伊藤英臣、石井聡、白鳥豊、大島健志郎、大塚重人 、服部正 平 、妹尾啓史
    • Organizer
      日本土壌肥料学会 2012 年度鳥取大会
    • Place of Presentation
      鳥取大学、鳥取市
    • Year and Date
      2012-09-05
  • [Presentation] Effect of vegetation cover on bacterial diversity of volcanic deposits in Miyake-jima2012

    • Author(s)
      Yong Guo, Reiko Fujimura, Yoshinori Sato, Wataru Suwata, Kenshiro Oshima, Masahira Hattori, Takashi Kamijo, Hiroyuki Ohta
    • Organizer
      Japan, 日本土壌肥料学会2012年度鳥取大会
    • Place of Presentation
      鳥取大学、鳥取市
    • Year and Date
      2012-09-04
  • [Presentation] Bacterial community of the fresh volcanic deposits on barren land, grassland, and woodland in the Island of Miyake, Japan, as revealed by pyrosequencing-based analysis of 16S rRNA genes2012

    • Author(s)
      Yong Guo, Reiko Fujimura, Yoshinori Sato, Kenshiro Oshima, Masahira Hattori, Takashi Kamijo, Hiroyuki Ohta
    • Organizer
      14th International Symposium on Microbial Ecology
    • Place of Presentation
      コペンハーゲン、デンマーク
    • Year and Date
      2012-08-21
  • [Presentation] Metagenomic analysis uncovered novel structure of microbial communities involved in denitrification in paddy soil2012

    • Author(s)
      Hideomi Itoh, Satoshi Ishii, Yutaka Shiratori, Kenshiro Oshima, Shigeto Otsuka, Masahira Hattori and Keishi Senoo
    • Organizer
      14th International Symposium on Microbial Ecology
    • Place of Presentation
      コペンハーゲン、 デンマーク
    • Year and Date
      2012-08-20
  • [Presentation] メタゲノム解析から見えた土壌圏の一酸化二窒素還元に関与する新たな微生物群集構造2012

    • Author(s)
      伊藤英臣、石井聡、大島健志郎、 大塚重人 、服 部正平 、妹尾啓史
    • Organizer
      日本土壌微生物学会2012年度神戸大会
    • Place of Presentation
      神戸大学、神戸市
    • Year and Date
      2012-06-23
  • [Presentation] Metagenomicand metatranscriptomic analysis of microbial communities in rice paddy soil2012

    • Author(s)
      Hideomi Itoh and Keishi Senoo
    • Organizer
      Japan-China International Symposium of Biotechnology onAgriculture and Environment
    • Place of Presentation
      東京大学、東京
    • Year and Date
      2012-05-13
  • [Presentation] 水田土壌微 生物群集のメタトランスクリプトーム解析2012

    • Author(s)
      伊藤英臣、石井聡、大島健志朗、白鳥豊、 大 塚重人、服部正 平 、妹尾啓史
    • Organizer
      第6回日本ゲノム微生物学会年会(東京)
    • Place of Presentation
      立教大学、東京
    • Year and Date
      2012-03-11
  • [Presentation] メタゲノ ム解析による水田脱窒機構の解明、農業環境 技術研究所公開セミナー2011

    • Author(s)
      妹尾啓 史 、吉田愛美、伊藤英臣
    • Organizer
      微生物ゲノム情報の活用による温室効果ガス研究の新展開
    • Place of Presentation
      つくば国際会議場、つくば市
    • Year and Date
      2011-12-06
  • [Presentation] 三宅島 2000 年噴火堆積物から分離された鉄 酸化細菌 Leptospirillum ferrooxidans C2-3 株の ゲノム解析2011

    • Author(s)
      藤村玲子,佐藤嘉則,細川研,西澤智康, 大島健志朗,服部正平,上條隆志,太田寛行
    • Organizer
      日本土壌微生物学会2011年度大会
    • Place of Presentation
      鳴子公民館、大崎市
    • Year and Date
      2011-11-26
  • [Presentation] Analysis of bacterial community in early stage of soil ecosystem after the 2000 volcanic eruption in Miyake-jima Island, Japan2011

    • Author(s)
      Yong Guo, Reiko Fujimura, Ken Hosokawa, Yoshinori Sato, Tomoyasu Nishizawa , TakashiKamijo and Hiroyuki Ohta
    • Organizer
      第26回日本微生物生態学会大会(つ くば)
    • Place of Presentation
      筑波大学
    • Year and Date
      2011-11-25
  • [Presentation] 三宅島 2000 年噴火火山灰堆 積物における初成土壌微生物生態系のメタゲノム解析2011

    • Author(s)
      藤村玲子,金錫元,郭永,佐藤嘉則,細川 研,須田亙,大島健志朗,服部正平,上條隆志,太田寛行
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都大学、京都市
    • Year and Date
      2011-10-09
  • [Presentation] Metagenomic analysis of microbial succession on fresh volcanic ash deposits in the Island of Miyake2011

    • Author(s)
      Reiko Fujimura, Seokwon Kim, Yong Guo, Yoshinori Sato, Ken Hosokawa, Wataru Suda, Erika Iioka, Kenshiro Ohshima, Masahira Hattori, Takashi Kamijo and Hiroyuki Ohta
    • Organizer
      Japan, 20th International Symposium on Environmental Biogeochemistry
    • Place of Presentation
      イスタンブール工科大学,イスタンブール
    • Year and Date
      2011-09-28
  • [Presentation] ACTIVEBACTERIAL AND ARCHAEAL COMMUNITIES RESPONDED TO WATERLOGGING OR DRAINAGE IN RICE PADDY SOIL, AS REVEALED BY COMPARATIVE LARGE-SCALE CLONE LIBRARY ANALYSIS2011

    • Author(s)
      Hideomi Itoh, Satoshi Ishii, Yutaka Shiratori, Kenshiro Oshima, Shigeto Otuka, Masahira Hattori, and Keishi Senoo
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌国際会議場、札幌
    • Year and Date
      2011-09-08
  • [Presentation] Relationship between development of bacterial community and vegetation succession on early soil ecosystem in the Island of Miyake, Japan2011

    • Author(s)
      郭永,藤村玲子,細川研,佐藤嘉則,西澤智康 , 上條隆志 , 太田寛行
    • Organizer
      日本土壌肥料学会 2011 年度つくば大会
    • Place of Presentation
      筑波大学、つくば市
    • Year and Date
      2011-08-09
  • [Presentation] Seasonal transition of active microbial communities in rice paddy soil, as revealed by metatranscriptomic approach2011

    • Author(s)
      Hideomi Itoh, Satoshi Ishii, Kenshiro Oshima, Yutaka Shiratori, Shigeto Otsuka, Masahira Hattori, and Keishi Senoo
    • Organizer
      11th Conference on Bacterial Genetics and Ecology
    • Place of Presentation
      Corfu, ギリシャ
    • Year and Date
      2011-06-01
  • [Presentation] Phylogenic andmetabolic analysis of microbial communities in paddy soil through comparative metagenomics2010

    • Author(s)
      Hideomi Itoh, Satoshi Ishii, Kenshiro Oshima, Yutaka Shiratori, Shigeto Otuka, Masahira Hattori, and Keishi Senoo
    • Organizer
      Soil Metagenomics 2010
    • Place of Presentation
      Braunschweig, Germany
    • Year and Date
      2010-12-09
  • [Presentation] 水田土壌のメタゲノム解析2010

    • Author(s)
      伊藤英臣、石井聡、大島健志朗、白鳥豊、 大 塚重人、服部正 平 、妹尾啓史
    • Organizer
      第26回日本微生物生態学 会大会(つくば)
    • Place of Presentation
      筑波大学
    • Year and Date
      2010-11-25
  • [Presentation] 農業環境分野へのメタゲノム解析の展開2010

    • Author(s)
      妹尾啓 史 ・石井聡・伊藤英臣
    • Organizer
      日本水産学会2010 年度秋季大会シンポジウム
    • Place of Presentation
      京都大学
    • Year and Date
      2010-09-25
  • [Presentation] Characterization of soil microbial community in the early soil ecosystem in Miyake Island, Japan2010

    • Author(s)
      Yong Guo, Reiko Fujimura, Ken Hosokawa, Yoshinori Sato, Tomoyasu Nishizawa, Takashi Kamijo, and Hiroyuki Ohta
    • Organizer
      日本土壌肥料学会2010年度札幌大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2010-09-07
  • [Presentation] MICROBIAL COMMUNITY STRUCTURE IN VOLCANICDEPOSITS WITH DIFFERENT SUCCESSION STAGES OF VEGETATION RECOVERY IN MIYAKE ISLAND, JAPAN2010

    • Author(s)
      Yong Guo, Reiko Fujimura, Ken Hosokawa, Yoshinori Sato, Tomoyasu Nishizawa, Takashi Kamijo, and Hiroyuki Ohta
    • Organizer
      13th International Symposium on Microbial Ecology
    • Place of Presentation
      Seattle, WA, USA
    • Year and Date
      2010-08-26
  • [Presentation] NITROGEN FIXATION BY IRON-OXIDIZING BACTERIA DURING EARLY ECOSYSTEM DEVELOPMENT ON MIYAKE-JIMA2010

    • Author(s)
      Reiko Fujimura, Yoshinori Sato, Tomonori Sugawara, Ken Hosokawa, Tomoyasu Nishizawa, Kenji Nanba, Takashi Kamijo, Bill Holben and Hiroyuki Ohta
    • Organizer
      JAPAN, 13th International Symposium on Microbial Ecology
    • Place of Presentation
      Seattle, WA, USA
    • Year and Date
      2010-08-26
  • [Presentation] SEASONAL TRANSITION OF BACTERIA, ARCHAEA, AND EUKARYOTE COMMUNITIES ALONG REDOX POTENTIAL CHANGES IN RICE PADDY SOIL2010

    • Author(s)
      Hideomi Itoh, Yutaka Shiratori, Satoshi Ishii, Shigeto Otsuka, Keishi Senoo
    • Organizer
      13th International Symposium on Microbial Ecology
    • Place of Presentation
      Seattle, WA, USA
    • Year and Date
      2010-08-25
  • [Book] 水田土壌のメタゲノム解析、In メタゲノム解析技術の最前線2010

    • Author(s)
      伊藤英臣、石井聡、妹尾啓史
    • Total Pages
      215-221
    • Publisher
      シーエムシー出版

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Published: 2014-08-29  

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