Study of the molecular mechanisms of synaptic plasticity and itsinvolvements in human diseases.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22300122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of Miyazaki
• Principal Investigator: TAKAMIYA Kogo 宮崎大学, 医学部, 教授 (40283767)
• Co-Investigator(Renkei-kenkyūsha): WAKAZONO Yoshihiko 宮崎大学, 医学部, 助教 (90377755)
• Project Period (FY): 2010 – 2012
• Keywords: シナプス可塑性 / グルタミン酸受容体 / 学習・記憶

Research Abstract

We studied the molecular mechanisms of synaptic plasticity in this project. Inparticular, we focused on the GRIP protein on AMPA receptor trafficking, which plays a key role insynaptic plasticity expression. It turned out that GRIP is located in exocyst and play an importantfunction in AMPA receptor cell surface expression. In addition, we have established a system toaccurately introduce exogenous gene into hippocampus to study synaptic plasticity in vivo.

Research Products

• [Journal Article] Involvement of AMPA Receptor GluR2 and GluR3Trafficking in Trigeminal Spinal Subnucleus Caudalis andC1/C2 Neurons in Acute-Facial Inflammatory Pain. (2012)
  • Author(s): Miyamoto M, Tsuboi Y, Honda K, Kobayashi M,Takamiya K, Huganir RL, Kondo M, Shinoda M, SessleBJ, Katagiri A, Kita D, Suzuki I, Oi Y, Iwata K.
  • Journal Title: PLoS One
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of synaptic plasticity underlyinglearning and memory (2011)
  • Author(s): Takamiya K.
  • Journal Title: Seikagaku Pages: 1016-1026
• [Journal Article] Involvement of GluR2 and GluR3 subunit C-termini inthe trigeminal spinal subnucleus caudalis and C1-C2neurons in trigeminal neuropathic pain. (2011)
  • Author(s): Miyamoto M,Tsuboi Y, Takamiya K, Huganir RL, Kondo M, Shinoda M,Oi Y, Iwata K.
  • Journal Title: Neuroscience letters Pages: 8-12
  • Peer Reviewed
• [Journal Article] Enhanced synaptic plasticity in mice withphosphomimetic mutation of the GluA1 AMPA receptor. (2011)
  • Author(s): Makino Y, Johnson RC, Yu Y, Takamiya K, Huganir RL.
  • Journal Title: Proc Natl Acad Sci U S A Pages: 8450-8455
  • Peer Reviewed
• [Journal Article] Phosphorylation of AMPA receptors is required forsensory deprivation-induced homeostatic synapticplasticity. (2011)
  • Author(s): Goel A, Xu LW, Snyder KP, Song L,Goenaga-Vazquez Y, Megill A, Takamiya K, Huganir RL,Lee HK.
  • Journal Title: PLoS One
  • Peer Reviewed
• [Journal Article] Developmental regulation of protein interacting with Ckinase 1 (PICK1) function in hippocampal synapticplasticity and learning. (2010)
  • Author(s): Volk L, Kim CH, Takamiya K, YuY, Huganir RL.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 21784-21789
  • Peer Reviewed
• [Journal Article] GRIP1 and 2 regulate activity-dependent AMPA receptorrecycling via exocyst complex interactions. (2010)
  • Author(s): Mao L,Takamiya K, Thomas G, Lin DT, Huganir RL.
  • Journal Title: Proceedings of the National Academy of Sciences of theUnited States of America Volume: 19038-19043
  • Peer Reviewed
• [Presentation] Organic and inorganic arsenic impairsynaptic potentials: Studies on GluA1trafficking. (2012)
  • Author(s): H. Madhyastha, M. Maruyama, K. Takamiya
  • Organizer: 北米神経科学界
  • Year and Date: 20120000
• [Presentation] LTP 誘導機構解明のための海馬 CA1 領域選択的、外来遺伝子導入法の確立とその適用Application of hippocampus CA1-selectivegene transfer system for analyzing LTPexpression mechanisms
  • Author(s): Y. Wakazono, J. Tsutajima, T. Kunitake, K.Takamiya
  • Organizer: 第35回日本神経科学大会
• [Remarks]
  • URL: http://www.med.miyazaki-u.ac.jp/1physiol/default.html