Modulation of rank-ordered recruitment of motor units in the jaw-closing movement by leak K+ channels

2013 Fiscal Year Final Research Report

• Project/Area Number: 22300127
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Osaka University
• Principal Investigator: KANG Youngnam 大阪大学, 歯学研究科(研究院), 教授 (50177755)
• Co-Investigator(Kenkyū-buntansha): TOYODA Hiroki 大阪大学, 大学院歯学研究科, 准教授 (00432451) ; SARO Hajjme 大阪大学, 大学院歯学研究科, 助教 (10432452) ; SAITO Mitsuru 大阪大学, 大学院歯学研究科, 講師 (50347770)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Keywords: 三叉神経運動ニューロン / 漏洩Kチャネル / NO / 序列動員

Research Abstract

The immunohistochemical analysis of motoneurons (MNs) in the trigeminal motor nucleus that innervates jaw-closing muscles revealed that TASK1 and TASK3 are complementary expressed in the soma and dendrites of MNs, respectively. The real-time RT PCR revealed that large MNs (>35 microm) expressed more TASK1/cell and TASK3 compared to small MNs (<20 microm). Consequently, larger MNs had smaller input resistance (IR) and more hyperpolarized resting membrane potentials (RMP) compared to smaller MNs. PKG activation enhanced TASK1 activity while it inhibited TASK3 activity. As the MN size becomes smaller, PKG activation more decreased the IR and hyperpolarized the RMP. The frequency distribution of sizes of alpha-MNs was found to display a bimodal distribution composed of smaller and larger alpha-MNs while gamma-MNs displayed a unimodal patter of smaller size. These finding provide crucial molecular and cellular bases of the rank-ordered recruitment of motor units in the jaw-closing movement.

Research Products

• [Journal Article] Enhanced desensitization followed by unusual resensitization in GABAA receptors in phospholipase C-related catalytically inactive protein-1/2 double-knockout mice (2014)
  • Author(s): Toyoda H, Saito M, Sato H, Tanaka T, Ogawa T, Yatani H, Kawano T, Kanematsu T, Hirata M, Kang Y
  • Journal Title: Pflugers Archiv - European Journal of Physiology Volume: (Epub ahead of print)
  • DOI: 10.1007/s00424-014-1511-5
  • Peer Reviewed
• [Journal Article] Teeth clenching reduces arm abduction force (2014)
  • Author(s): Sato H, Kawano T, Saito M, Toyoda H, Maeda Y, Tiirker KS, Kang Y
  • Journal Title: Experimental Brain Research Volume: (Epub ahead of print)
  • DOI: 10.1007/s00221-014-3919-8
  • Peer Reviewed
• [Journal Article] A mathematical model of negative covariability of inter-columnar excitatory synaptic actions caused by presynaptic inhibition (2013)
  • Author(s): Saito M, Tanaka T, Sato H, Toyoda H, Aoyagi T, Kang Y
  • Journal Title: The European Journal of euroscience Volume: 38 Pages: 2999-3007
  • DOI: 10.1111/ejn.12299
  • Peer Reviewed
• [Journal Article] GABAB receptor-mediated presynaptic inhibition reverses inter-columnar covariability of synaptic actions by intracortical axons in the rat barrel cortex (2013)
  • Author(s): Sato H, Toyoda H, Saito M, Kobayashi M, Althof D, Kulik A, Kang Y
  • Journal Title: The European Journal of Neuroscience Volume: 37 Pages: 190-202
  • DOI: 10.1111/ejn.12041
  • Peer Reviewed
• [Journal Article] Illusion caused by vibration of muscle spindles reveals an involvement of muscle spindle inputs in regulating isometric contraction of masseter muscles (2012)
  • Author(s): Tsukiboshi T, Sato H, Tanaka Y, Saito M, Toyoda H, Morimoto T, Tiirker KS, Maeda Y, Kang Y
  • Journal Title: Journal of Neurophysiology Volume: 108 Pages: 2524-2533
  • DOI: 10.1152/jn.00997.2011
  • Peer Reviewed
• [Journal Article] Capsaicin induces theta-band synchronization between gustatory and autonomic insular cortices (2012)
  • Author(s): Saito M, Toyoda H, Kawakami S, Sato H, Bae YC, Kang Y.
  • Journal Title: The Journal of Neuroscience Volume: 32 Pages: 13470-13487
  • DOI: 10.1523/JNEUROSCI.5906-11.2012
  • Peer Reviewed
• [Journal Article] 口腔領域をめぐる神経基盤】噛みしめ運動における咬筋運動ニューロン序列動員の階層性制御機構 (2011)
  • Author(s): 姜 英男,齋藤 充,豊田 博紀,佐藤 元
  • Journal Title: 脳 Volume: 21,14 Pages: 385-391
  • URL: http://mol.medicalonline.jp/library/journal/download?GoodslD=ac5nonid/2011/001404/018&name=0385-0391j&basejamas_pdf
• [Journal Article] Protein kinase G dynamically modulates TASK1-mediated leak K+ currents in cholinergic neurons of the basal forebrain (2010)
  • Author(s): Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang, Y
  • Journal Title: The Journal of Neuroscience Volume: 30 Pages: 5677-5689
  • DOI: 10.1523/JNEUROSCL5407-09.2010
  • Peer Reviewed
• [Journal Article] Classification of extracellularly recorded neurons by their discharge patterns and their correlates with intracellularly identified neuronal types in the frontal cortex of behaving monkeys (2010)
  • Author(s): Katai S, Kato K, Ueno S, Kang Y, Saruwatari M, Ishikawa N, Inoue M, Mikami A
  • Journal Title: European Journal of Neuroscience Volume: 31 Pages: 1322-1338
  • DOI: 10.1111/j.1460-9568.2010.07150.x
  • Peer Reviewed
• [Journal Article] Selectively targeting pain in the trigeminal system (2010)
  • Author(s): Kim HY, Kim K, Li HY, Chung G, Park CK, Kim JS, Jung SJ, Lee MK, Ahn DK, Hwang SJ, Kang Y, Binshtok AM, Bean BP, Woolf CJ, Oh SB
  • Journal Title: Pain Volume: 150 Pages: 29-40
  • DOI: 10.1016/j.pain.2010.02.016
  • Peer Reviewed
• [Journal Article] Rank-ordered recruitment of masseter motoneurons by the activity of mesencephalic trigeminal neurons during slow closing phase of mastication cycle (2010)
  • Author(s): Kang Y, Saito M, Toyoda H, Sato H
  • Journal Title: Journal of Oral Bioscience Volume: 52 Pages: 330-335
  • URL: http://mol.medicalonline.jp/library/journal/download?GoodslD=ct4biosc/2010/005204/005&name=0330-0335e&basejamaspdf
• [Presentation] Phospholipase C-related inactive proteins are involved in the regulation of phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda H, Saito M, Sato H, Kanematsu T, Hirata M and Kang Y
  • Organizer: The 43rd Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2013-11-11
• [Presentation] Rank-ordered recruitment of jaw-closing a-motoneurons depending on the activities of TASK1 and TASK3 channels in the rat (2013)
  • Author(s): Saito M, Isogai-Morita Y, Emura N, Toyoda H and Kang Y
  • Organizer: The 43rd Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2013-11-11
• [Presentation] カプサイシンの島皮質での受容による内臓―内臓問自律神経反射の修飾の可能性 (2013)
  • Author(s): 齋藤 充,豊田 博紀,佐藤 元,姜 英男
  • Organizer: 日本味と匂学会第47回大会
  • Place of Presentation: 仙台
  • Year and Date: 2013-09-07
• [Presentation] Phospholipase C-related inactive protein regulates the phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda H, Saito M, Sato H, Kanematsu T, Hirata M and Kong Y
  • Organizer: The 11 th Japan-Korea Joint Symposium of Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: Shizuoka(.国際学会―一般講演)
  • Year and Date: 2013-09-06
• [Presentation] Phospholipase C-related inactive protein modulates desensitization and resensitization of GABAA receptor currents in the barrel cortex (2012)
  • Author(s): Toyoda H, Ogawa T, Saito M, Sato H, Kanematsu M, Hirata M, Kang Y
  • Organizer: The 42nd Annual Meeting of the Society for Neuroscience
  • Place of Presentation: New Orleans
  • Year and Date: 2012-10-14
• [Presentation] A physiological method to determine the vertical dimension of occlusion (2012)
  • Author(s): Tanaka Y, Sato H, Saito M, Tsukiboshi T, Maeda Y, Kang Y
  • Organizer: Society for Neuroscience The 42nd Annual Meeting of the Society for Neuroscience
  • Place of Presentation: New Orleans
  • Year and Date: 2012-10-14
• [Presentation] cGMP differentially modulates leak K^<+> currents in masseter motoneurons (2011)
  • Author(s): Toyoda H, Hirao K, Saito M, Sato H, Kang Y
  • Organizer: The 41st Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington, USA
  • Year and Date: 2011-11-13
• [Presentation] 噛みしめ運動から見た運動ニューロン序列動員の階層的制御機構―漏洩K^<+>チャネル分子―伸張反射回路―小脳内部モデル― (2011)
  • Author(s): 姜 英男
  • Organizer: 大阪大学MEIセンターグローバルCOE"in silico medicine"第13回定例シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-03-03
• [Presentation] Membrane hyperpolarization depresses AMPA currents in the primary sensory neuron of the rat mesencephalic trigeminal nucleus (2010)
  • Author(s): Kawasaki Y, Saito M, Toyoda H, Sato H, Tanaka S, Kogo M, Kang Y
  • Organizer: The 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego.(国内学会―シンポジウム)
  • Year and Date: 2010-11-17
• [Presentation] Protein kinase G (PKG) bidirectionally modulates TASK1 currents in PKG-loaded HEK 293 cells (2010)
  • Author(s): Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang Y
  • Organizer: The 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-15
• [Presentation] Electrophysiological properties of capsaicin-induced currents in layer II/III and layer V pyramidal cells of the insular cortex (2010)
  • Author(s): Sato H, Kawakami S, Toyoda H, Saito M, Bae YC, Kong Y
  • Organizer: The 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-15
• [Presentation] Inter- and intracolumnar desynchronization by presynaptic GABAB inhibition in the rat barrel cortex (2010)
  • Author(s): Saito M, Sato H, Toyoda H, Kobayashi M, Althof D, Kulik A, Shigemoto R, Kang Y
  • Organizer: The 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-15
• [Book] Recruitment of masseter motoneurons by the presumed spindle Ia inputs. In: Breathe, Walk and Chew - The Neural Challenge: Part I (Progress in Brain Research, Vol. 187)(Gossard JP, Dubuc R, Kolta A, Eds.)(163-171) (2010)
  • Author(s): Kang Y, Saito M, Toyoda H, Sato H
  • Publisher: Elsevier, Amsterdam
• [Book] Recruitment of masseter motoneurons by spindle Ia inputs and its modulation by leak K^<+> channels. In. Interface Oral Health Science 2009 (SasanO T, Suzuki O, Eds.)(60-65) (2010)
  • Author(s): Kang Y, Toyoda H, Saito M, Sato H.
  • Publisher: Springer, Tokyo
  • URL: http://link.springer.com/chapter/10.1007/978-4-431-99644-6_9
• [Remarks]
  • URL: http://web.dent.osaka-u.ac.jp/～phys/