2014 Fiscal Year Final Research Report
Development of a new single-cell imaging method using oxonol voltage-sensitive dyes
| Project/Area Number |
22300129
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Kanto Gakuin University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Katsushige 駒沢女子大学, 人間健康学部, 教授 (80291342)
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Keywords | 膜電位感受性色素 / イメージング / 鶏胚 / 細胞 |
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| Outline of Final Research Achievements |
We evaluated the suitability of voltage-sensitive oxonol dyes for optical imaging at the single-cell level. We screened four oxonol dyes in dissociated cells from 8-9 day-old embryonic chick brainstems. Voltage-related fluorescent changes in response to the perfusion of a high-K+ solution were measured using a cooled CCD camera. Clear optical responses were detected with every dye, suggesting the potential utility of the oxonol dye in optical imaging of voltage changes at the single-cell level.
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| Free Research Field |
生理学
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