2012 Fiscal Year Final Research Report
Molecular mechanisms of the sleep control by neurons activated during sleep
| Project/Area Number |
22300133
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fusional basic brain science
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| Research Institution | 公益財団法人大阪バイオサイエンス研究所 (2012)
Osaka Bioscience Institute (2010-2011) |
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Principal Investigator |
URADE Yoshihiro 公益財団法人大阪バイオサイエンス研究所, 分子行動生物学部門, 研究部長 (10201360)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMORI Ko 大阪薬科大学, 薬学部, 講師 (70425453)
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| Co-Investigator(Renkei-kenkyūsha) |
KASHIWAGI Kaori (公財)大阪バイオサイエンス研究所, 分子行動生物学部門, 研究員 (10372666)
NAGATA Nanae (公財)大阪バイオサイエンス研究所, 分子行動生物学部門, 研究員 (80390805)
ZHI Li-huang (公財)大阪バイオサイエンス研究所, 分子行動生物学部門, 研究副部長 (10321704)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 睡眠 / Sox5 |
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| Research Abstract |
In this study, we identified a novel splicing isoform of Sox5 with truncation at exon 2, Sox5-t2. Q-PCR analysis revealed that Sox5-t2 was dominantly expressed in the adult mouse cortex. Electrophoretic mobility shift assay showed that Sox5-t2 binds to genes that are up-regulated during sleep and contain Sox5-binding elements. By conditional knockout of Sox5 in the forebrain, the amount of REM sleep decreased in the light phase. These results suggest that Sox5 is an important regulator of sleep-related functions of neurons.
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