In vivo analysis of cerebellar granular cell networks using a micro-endoscope

2013 Fiscal Year Final Research Report

• Project/Area Number: 22300136
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fusional brain recording science
• Research Institution: Osaka Bioscience Institute
• Principal Investigator: FUNABIKI Kazuo 公益財団法人大阪バイオサイエンス研究所, システムズ生物学, 研究副部長 (00301234)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: 小脳 / 顆粒細胞 / 顕微内視鏡 / 情報処理 / 学習理論

Research Abstract

We developed a micro-endoscope system which enables in vivo Ca imaging of neural circuits located deep brain area at cellular resolution in head-restricted non-anesthetized mice. We applied this system to the analysis of the cerebellar granule cell networks. We used mutants in which Ca sensor protein (GCaMP2) is expressed only in the cerebellar granule cell layers (Knopfel et al). First, we explored the role of synaptic transmission from the cerebellar granule cells in reflexive eye movements, by using a mutant in which synaptic transmission from cerebellar granule cells can be reversibly blocked in Dox dependent manner(Wada et al, 2014). Next, we conducted in vivo Ca imaging experiments in the cerebellar flocculus, which is known to contribute to the control of reflexive eye movement, and could visualize Ca response of cerebellar granule cells of the flocculus in vivo with physiological stimuli.

Research Products

• [Journal Article] Aversive behavior induced by optogenetic inactivation of ventral tegmental area dopamine neurons is mediated by dopamine D2 receptors in the nucleus accumbens (2014)
  • Author(s): Danjo T, Yoshimi K, Funabiki K, Yawata S, Nakanishi S
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111(17) Pages: 6455-60
  • Peer Reviewed
• [Journal Article] Role of granule-cell transmission in memory trace of cerebellum-dependent optokinetic motor learning (2014)
  • Author(s): Wada N, Funabiki K, Nakanishi S
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111(14) Pages: 5373-8
  • Peer Reviewed
• [Journal Article] Theoretical foundations of the sound analog membrane potential that underlies coincidence detection in the barn owl (2013)
  • Author(s): Ashida G, Funabiki K, Carr CE
  • Journal Title: Front Comput Neurosci Volume: 7 Pages: 151
  • Peer Reviewed
• [Journal Article] Biophysical basis of the sound analog membrane potential that underlies coincidence detection in the barn owl (2013)
  • Author(s): Ashida G, Funabiki K, Carr CE
  • Journal Title: Front Comput Neurosci Volume: 7 Pages: 102
  • Peer Reviewed
• [Journal Article] Benign paroxysmal positional vertigo and head position during sleep (2012)
  • Author(s): Shigeno K, Ogita H, Funabiki K
  • Journal Title: J Vestib Res Volume: 22(4) Pages: 197-203
  • Peer Reviewed
• [Journal Article] Signal-to-noise ratio in the membrane potential of the owl's auditory coincidence detectors (2012)
  • Author(s): Ashida G, Funabiki K, Kuokkanen PT, Kempter R, Carr CE
  • Journal Title: J Neurophysiol Volume: 108(10) Pages: 2837-45
  • Peer Reviewed
• [Journal Article] Spatio-temporal control of neural activity in vivo using fluorescence microendoscopy (2012)
  • Author(s): Hayashi Y, Tagawa Y, Yawata S, Nakanishi S, Funabiki K
  • Journal Title: Eur J Neurosci Volume: 36(6) Pages: 2722-32
  • Peer Reviewed
• [Journal Article] Computation of interaural time difference in the owl's coincidence detector neurons (2011)
  • Author(s): Funabiki K, Ashida G, Konishi M
  • Journal Title: J Neurosci Volume: 31(43) Pages: 15245-56
  • Peer Reviewed
• [Journal Article] Clinical and epidemiological study on inpatients with vertigo at the ENT Department of Kyoto University Hospital (2010)
  • Author(s): Ogita H, Taura A, Funabiki K, Miura M, Ito J
  • Journal Title: Acta Otolaryngol Suppl Volume: 563 Pages: 34-8
  • Peer Reviewed
• [Journal Article] Clinical study of vertigo in the outpatient clinic of Kyoto University Hospital (2010)
  • Author(s): Taura A, Ohgita H, Funabiki K, Miura M, Naito Y, Ito J
  • Journal Title: Acta Otolaryngol Suppl Volume: 563 Pages: 29-33
  • Peer Reviewed
• [Journal Article] Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior (2010)
  • Author(s): Hikida T, Kimura K, Wada N, Funabiki K, Nakanishi S
  • Journal Title: Neuron Volume: 66(6) Pages: 896-907
  • Peer Reviewed
• [Journal Article] Protein kinase G dynamically modulates TASK1-mediated leak K+ currents in cholinergic neurons of the basal forebrain (2010)
  • Author(s): Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang Y
  • Journal Title: J Neurosci Volume: 30(16) Pages: 5677-89
  • Peer Reviewed
• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade (2010)
  • Author(s): Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107(1) Pages: 332-7
  • Peer Reviewed
• [Presentation] Development of micro-endoscope for in vivo Ca and FRET imaging (2014)
  • Author(s): Ichiro Nakahara, Akihiro Goto, Kazuo Funabiki
  • Organizer: ARO midwinter meeting
  • Place of Presentation: San Diego
  • Year and Date: 2014-02-22
• [Presentation] Computation of Interaural Time Difference in the Owl's Auditory Coincidence Detector Neurons (2012)
  • Author(s): Kazuo Funabiki, Go Ashida, Masakazu Konishi
  • Organizer: 35th The Association for Research in Otolaryngology
  • Place of Presentation: SanDiego、USA
  • Year and Date: 2012-02-25
• [Presentation] How the owl's coincidence detectors work? (2011)
  • Author(s): Kazuo Funabiki
  • Organizer: 16th Auditory Research Form
  • Place of Presentation: 滋賀
  • Year and Date: 2011-12-02