2013 Fiscal Year Final Research Report
Design of Scaffold Materials Bearing Stabilization of Gwoth Factors Toward Angiogenesis
| Project/Area Number |
22300165
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kobe University |
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Principal Investigator |
OOYA Tooru 神戸大学, 工学(系)研究科(研究院), 准教授 (10301201)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 成長因子 / 安定化 / デンドリマー / 水の構造 / 細胞 / 疎水場 / ポリグリセロール |
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| Research Abstract |
In this project, polyglycerol dendrimers (PGDs) were focused as a candidate of protein stabilizers aiming at creation of new polymeric scaffolds bearing growth factor stabilization. Thermal denatulation of alcohol dehydrogenase (ADH), as a model protein, was found to be prevented in the presence of PGDs, suggesting that the PGDs act as a stabilizer of proteins. This effect was due to modulation of water structure, which was clarified by NMR studies. In addition, PGDs could interact with basic amino acids, which was investigated by isothermal titration calorimetric studies, and biological activity of basic fibroblast growth factor (bFGF) was apparently preserved by the addition of PGDs. Therefore, PGDs are feasible to use as a protein stabilizer, and can be applicable as a polymeric scaffold bearing growth factor stabilization.
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