The microRNAsfor the diagnosis and treatment of uppergastro-intestinal malignancy - application of appatite-nanocarrier -

2012 Fiscal Year Final Research Report

• Project/Area Number: 22300169
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Keio University
• Principal Investigator: SUZUKI Hidekazu 慶應義塾大学, 医学部, 准教授 (70255454)
• Co-Investigator(Kenkyū-buntansha): SAITO Yoshimasa 慶應義塾大学, 薬学部, 准教授 (90360114) ; AKAIKE Toshihiro 東京工業大学, 生命理工学部, 教授 (30101207) ; KANAI Yae 国立がん研究センター, 研究所, 副所長 (00260315) ; ONISHI Yasuyuki 実験動物中央研究所, 研究員 (70201382)
• Project Period (FY): 2010 – 2012
• Keywords: マイクロRNA / アパタイトナノキャリア / がん遺伝子 / 胃がん / 担がん動物 / 細胞内導入

Research Abstract

The microRNAs (miRNAs) regulate both tumor development and progression by acting on tumor promoter or suppressor genes. Here, a delivery mechanism to introduce miRNA into the cytoplasm by using apatite nanoparticle is applied. 1) An miRNA microarray analy

Research Products

• [Journal Article] Helicobacter pyloriinfection in functional dyspepsia. (2013)
  • Author(s): Suzuki, H., Moayyedi, P.
  • Journal Title: Nat. Rev. Gastroenterol. Hepatol. Volume: 10(3) Pages: 168-74
  • DOI: DOI:10.1038/nrgastro.2013.9
  • Peer Reviewed
• [Journal Article] The role of microRNAin gastric malignancy. (2013)
  • Author(s): Nishizawa, T., Suzuki, H
  • Journal Title: Int. J. Mol. Sci. Volume: 14(5) Pages: 9487-9496
  • DOI: DOI:10.3390/ijms14059487
  • Peer Reviewed
• [Journal Article] The tumor suppressor microRNA-29cis downregulated and restored by celecoxib in human gastric cancer cells. (2013)
  • Author(s): Saito, Y., Suzuki, H., Kanai, Y., et al.
  • Journal Title: Int. J. Cancer Volume: 132(8) Pages: 1751-60
  • DOI: DOI:10.1002/ijc.27862.
  • Peer Reviewed
• [Journal Article] Roles of oxidative stress in stomach disorders. (2012)
  • Author(s): Suzuki, H., et al.
  • Journal Title: J. Clin. Biochem. Nutr. Volume: 50(1) Pages: 35-39
  • DOI: DOI:10.3164/jcbn.11-115SR
  • Peer Reviewed
• [Journal Article] FecA1, a bacterial iron transporter, determines the survival of Helicobacter pyloriin the stomach. (2012)
  • Author(s): Tsugawa, H., Suzuki, H., et al.
  • Journal Title: Free Radic. Biol. Med. Volume: 52(6) Pages: 1013-1010
  • DOI: DOI:10.1016/j.freeradbiomed.2011.12.011.
  • Peer Reviewed
• [Journal Article] Low-density lipoprotein receptorrelated protein-1 (LRP-1) mediates autophagy and apoptosis caused by Helicobacter pyloriVacA (2012)
  • Author(s): Yahiro, K., Suzuki, H., et al.
  • Journal Title: J. Biol. Chem Volume: 287(37) Pages: 31104-15
  • DOI: DOI:10.1074/jbc.M112.387498
  • Peer Reviewed
• [Journal Article] Development of a novel microRNApromoter microarray for ChIP-on-chip assay to identify epigenetically regulated microRNAs (2012)
  • Author(s): Saito, Y., Suzuki, H., et al.
  • Journal Title: Biochem. Biophys. Res. Com Volume: 426(1) Pages: 33-37
  • DOI: DOI:10.1016/j.bbrc.2012.08.012
  • Peer Reviewed
• [Journal Article] Overexpression of miR-142-5pand miR-155in gastric mucosa-associated lymphoid tissue (MALT) lymphoma resistant to Helicobacter pylori eradication (2012)
  • Author(s): Saito, Y., Suzuki, H., et al.
  • Journal Title: PLoS One Volume: 7(11) Pages: e47396
  • DOI: DOI:10.1371/journal.pone.0047396
  • Peer Reviewed
• [Journal Article] Helicobacter pyloriCagA degradation by reactive oxygen species-mediated autophagy is escaped in CD44 variant-expressing cancer stem-like cells (2012)
  • Author(s): Tsugawa, H., Suzuki, H., et al.
  • Journal Title: Cell Host Microbe. Volume: 12(6) Pages: 746-777
  • DOI: DOI:10.1016/j.chom.2012.10.014
  • Peer Reviewed
• [Journal Article] CDX1 confers intestinal phenotype on gastric epithelial cells via induction of stemness-associated reprogramming factors SALL4 and KLF5 (2012)
  • Author(s): Fujii, Y., Suzuki, H., et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109(50) Pages: 20584-20589
  • DOI: DOI:10.1073/pnas.1208651109
  • Peer Reviewed
• [Journal Article] Two amino acids mutation of Ferric uptake regulator (Fur) determines Helicobacter pylori resistance to metronidazole. (2011)
  • Author(s): Tsugawa, H., Suzuki, H., Saito, Y., et al.
  • Journal Title: Antioxid. Redox Signal Volume: 14(1) Pages: 15-23
  • DOI: DOI:10.1089/ars.2010.3146
  • Peer Reviewed
• [Journal Article] Dysfunctional gastric emptying with downregulation of muscle-specific microRNAs in Helicobacter pylori -infected mice. (2011)
  • Author(s): Saito, Y., Suzuki, H., et al.
  • Journal Title: Gastroenterology Volume: 140(1) Pages: 189-198
  • DOI: DOI:10.1053/j.gastro.2010.08.044.
  • Peer Reviewed
• [Journal Article] Etiological difference between ultrashort-segment Barrett's esophagus and short-segment Barrett's esophagus in Japan (2011)
  • Author(s): Matsuzaki, J., Suzuki, H., Saito, Y., et al.
  • Journal Title: J. Gastroenterol. Volume: 46(3) Pages: 332-8
  • DOI: DOI:10.1007/s00535-010-0353-y.
  • Peer Reviewed
• [Journal Article] Extragastric manifestations of Helicobacter pylori infection. (2011)
  • Author(s): Suzuki, H., et al.
  • Journal Title: Helicobacter Volume: 16(Suppl.1) Pages: 65-69
  • DOI: DOI:10.1111/j.1523-5378.2011.00883.x.
  • Peer Reviewed
• [Journal Article] Effect of lansoprazole versus roxatidine on prevention of bleeding and ulcer healing after endoscopic submucosal dissection for superficial gastric neoplasia (2011)
  • Author(s): Imaeda, H., Suzuki, H., Saito, Y., et al.
  • Journal Title: J. Gastroenterol. Volume: 46(11) Pages: 1267-72
  • DOI: DOI:10.1007/s00535-011-0447-1.
  • Peer Reviewed
• [Journal Article] MicroRNAs in hepatobiliary and pancreatic cancers. (2011)
  • Author(s): Saito, Y., Suzuki, H., et al.
  • Journal Title: Front. Gene. Volume: 2 Pages: 66
  • DOI: DOI:10.3389/fgene.2011.00066.
  • Peer Reviewed
• [Journal Article] Gallstones increase the prevalence of Barrett's esophagus. (2010)
  • Author(s): Matsuzaki, J., Suzuki, H., Saito, Y., et al.
  • Journal Title: J. Gastroenterol. Volume: 45(2) Pages: 171-8
  • DOI: DOI:10.1007/s00535-009-0153-4
  • Peer Reviewed
• [Journal Article] Contribution of efflux pumps to clarithromycin resistance in Helicobacter pylori (2010)
  • Author(s): Hirata, K., Suzuki, H., Saito, Y., et al.
  • Journal Title: J. Gastroenterol. Hepatol. Volume: 25(s1) Pages: S75-79
  • DOI: DOI:10.1111/j.1440-1746.2009.06220.x.
  • Peer Reviewed
• [Presentation] Dysfunctional gastric emptying with downregulation of muscle-specific microRNAs in Helicobacter pylori -infected mice. (2012)
  • Author(s): Saito, Y., Suzuki, H., et al.
  • Organizer: The 2nd Asian Pacific Topic Conference, Asian Pacific Helicobacter pylori Meeting 2012
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 20120113-15
• [Presentation] Resistance to oxidative stress is a principal pathway for the survival and disease development of Helicobacter pylori. "Helicobacter pylori and Tissue Injury" (2012)
  • Author(s): Suzuki, H., et al.
  • Organizer: 7th International Symposium on Cell/Tissue Injury and Cytoprotection/Organoprotection
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2012-09-11
• [Presentation] Helicobacter pyloriand gastric cancer. Symposium 7 "Helicobacter pylori-Pathogenesis & Clinical Manifestation-" (2012)
  • Author(s): Suzuki, H.
  • Organizer: The 14th Meeting of International Conference on Ulcer Research (ICUR)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2012-07-13
• [Presentation] Specific amino acid mutation of p53 enhanced the stability of intracellular cagAby inhibiting autophagy induction. (2012)
  • Author(s): Tsugawa, H., Suzuki, H., et al.
  • Organizer: AGA Research Forum, DDW2012
  • Place of Presentation: San Diego, USA
  • Year and Date: 2012-05-12
• [Presentation] Proliferation of cdx2-expressing human esophageal cells is enhanced by bile acid with upregulation. (2011)
  • Author(s): Matsuzaki, J., Suzuki, H., Saito, Y., and Akaike, T., et al.
  • Organizer: UEGW2011
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2011-10-25
• [Presentation] Activation of the tumor suppressor microRNA-29cby the selective cyclooxygenase-2 inhibitor, celecoxib in human gastric cancer. (2011)
  • Author(s): Suzuki, H., Saito, Y., Kanai, Y., et al
  • Organizer: AGA Research Forum DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-09
• [Presentation] H. pylori-Derived Oncoprotein, CagA, is Degraded by Autophagy in Human Gastric Epithelial Cells. (2011)
  • Author(s): Tsugawa, H., Suzuki, H., et al
  • Organizer: AGA Research Forum DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-07
• [Presentation] Enhanced Expression of MicroRNA-142-5p and MicroRNA-155 and Suppressed Expression of Tp53inp1 Would Be Novel Molecular Biomarkers for Gastric Mucosa-Associated Lymphoid Tissue (MALT) Lymphoma, Resistant to H. pylori Eradication. (2011)
  • Author(s): Suzuki, H., Saito, Y., et al.
  • Organizer: AGA Research Forum DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-07
• [Presentation] Gastric Cancer Screening in Japanese (2010)
  • Author(s): Suzuki, H.
  • Organizer: ASGE Clinical Symposium DDW2010
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20100501-05
• [Presentation] Overexpression of miR-142-5pand miR-155and suppression of tumor protein 53-induced nuclear protein 1 in gastric mucosa-associated lymphoid tissue lymphoma. (2010)
  • Author(s): Saito, Y., Suzuki, H., et al.
  • Organizer: AGA Research Forum DDW2010
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20100501-05
• [Presentation] Chronic infection with Helicobacter pylori induces downregulation of muscle-specific microRNAs and smooth muscle hypertrophy of the mouse stomach. (2010)
  • Author(s): Saito, Y., Suzuki, H., et al
  • Organizer: AGA Research Forum DDW2010
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20100501-05
• [Presentation] Induction of H. pylori resistance to metronidazole through enhanced expression of a bacterial efflux pump (2010)
  • Author(s): Tsugawa, H., Suzuki, H.Saito, Y., et al
  • Organizer: AGA Research Forum DDW2010
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20100501-05
• [Presentation] The role of the Ferric uptake regulator (Fur) in Helicobacter pylori resistance to metronidazole. (2010)
  • Author(s): Tsugawa, H., Suzuki, H., Saito, Y., et al.
  • Organizer: AGA Research Forum DDW2010
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20100501-05
• [Presentation] Caudal-related homeobox 2 (Cdx2) suppresses stratification of esophageal squamous epithelial cells by down-regulating microRNA-221/222 (2010)
  • Author(s): Matsuzaki, J., Suzuki, H., Saito, Y., et al.
  • Organizer: DDW2010
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20100501-05
• [Presentation] Downregulation of the tumor suppressor microRNA-29cplays a critical role in the progression of human gastric cancer. (2010)
  • Author(s): Saito, Y., Suzuki, H., et al.
  • Organizer: DDW2010
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20100501-05
• [Presentation] Upregulation of oncogenic microRNA-21followed by the downregulation of the tumor suppressor microRNA-29cin the progression of human gastric cancer. (2010)
  • Author(s): Suzuki, H., Saito, Y., Kanai, Y., et al
  • Organizer: UEGW2010
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-10-25
• [Presentation] Gastric cancer development - from the aspects of sonic hedgehog and microRNAs-. (2010)
  • Author(s): Suzuki, H.
  • Organizer: Gachon International Gastric Cancer Symposium 2010
  • Place of Presentation: Incheon, Korea
  • Year and Date: 2010-10-09
• [Presentation] Nitric oxide involvement in gastric disorders. (2010)
  • Author(s): Suzuki, H. (invited), Saito, Y., et al
  • Organizer: Symposium SP-04 "Nitrosative stress in the gastrointestinal tract", The 6thInternational Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-06-15
• [Book] Free Radical Biology in Digestive Diseases (2011)
  • Author(s): Suzuki, H., Nishizawa, T., Tsugawa, H., Hibi, T.(eds.) Naito, Y., Suematsu, M., Yoshikawa, T., Karger, Basel
  • Total Pages: 111-120
  • Publisher: Front. Gastrointest. Res.
• [Book] Chapter 5: MicroRNAs in Gastric Cancer. MicroRNAs in Cancer Translational Research (2011)
  • Author(s): Suzuki, H., Saito, Y., Hibi, T.(eds.) William C.S. Cho
  • Total Pages: 135-143
  • Publisher: Springer