2013 Fiscal Year Final Research Report
Basic investigation for regeneration using mesenchymal stem cells with multi-potency and immunological tolerance
| Project/Area Number |
22300185
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Nagoya University |
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Principal Investigator |
TORIHASHI Shigeko 名古屋大学, 医学(系)研究科(研究院), 教授 (90112961)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Hitoshi 名古屋大学, 大学院医学系研究科, 教授 (80173243)
KAWABE Tsutomu 名古屋大学, 大学院医学系研究科, 教授 (20378219)
ISHIDA Kazuto 名古屋大学, 大学院医学系研究科, 講師 (10303653)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 間葉系幹細胞 / 骨格筋 / 再生 / 移植 / ES細胞 / iPS細胞 / CD105 |
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| Research Abstract |
We developed mesenchymal stem cells (MSCs) expressing CD105 from mouse ES cells during induction to adipose cells. We investigated MSCs and obtained valuable results as follows: (1) Our MSCs showed higher telomerase activity than that of tissue MSCs. However,their immunological tolerance was not high enough for transplantation across species. (2) MSCs showed more than 96% of purity and laser irradiation to unlabeled cells improved the purity. (3) MSCs demonstrated high potential for differentiation into skeletal muscle and their transplantation intoinjured muscles accelerated functional recovery of damaged muscles. (4) Injured skeletal muscles cells up-regulated the expression of TSG6 in MSCs. It formed the foothold of MSCs after transplantation. (5) We established iPS cells from MSCs in human metabolic syndrome model rats. Adipose cells from the iPS cells showed pathophysiological state similar to human metabolic syndrome.
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