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2012 Fiscal Year Final Research Report

Regulatory mechanisms of translesion DNA synthesis

Research Project

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Project/Area Number 22310034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionNagoya University

Principal Investigator

MASUTANI Chikahide  名古屋大学, 環境医学研究所, 教授 (40241252)

Project Period (FY) 2010 – 2012
Keywords修復 / 損傷乗り越えDNA複製
Research Abstract

Human DNA polymerase eta (Polη) is the responsible gene product of a cancer prone genetic disorder, xeroderma pigmentosum variant, catalyzes accurate and efficient translesion DNA synthesis, and prevents cell death and carcinogenesis induced by DNA damages. This project revealed that human Polηhas a unique structure to catalyze the accurate and efficient translesion DNA synthesis. This project also identified a novel mechanism of PCNA ubiquitylation which is crucial for regulation of translesion DNA synthesis and related mechanisms.

  • Research Products

    (40 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (31 results) Remarks (1 results)

  • [Journal Article] Human DNA polymerase ηand its regulatory mechanisms.2012

    • Author(s)
      Masutani C.
    • Journal Title

      Genes Environ.

      Volume: 34 (2) Pages: 63-69

    • Peer Reviewed
  • [Journal Article] En bloctransfer of poly-ubiquitin chains to PCNA in vitrois mediated by two human E2-E3 pairs.2012

    • Author(s)
      Masuda Y., Suzuki M., Kawai H., Hishiki A., Hashimoto H., Masutani C., Hishida T., Suzuki F., *Kamiya K.
    • Journal Title

      Nucleic Acids Res.

      Volume: 40(20) Pages: 10394-10407

    • Peer Reviewed
  • [Journal Article] Photosensitized [2 + 2] cycloaddition of N-acetylated cytosine affords stereoselective formation of cyclobutane pyrimidine dimer.2011

    • Author(s)
      Yamamoto J., Nishiguchi K., Manabe K., Masutani C., Hanaoka F., Iwai S.
    • Journal Title

      Nucleic Acids Res

      Volume: 39 Pages: 1165-1175

    • Peer Reviewed
  • [Journal Article] Molecular Chaperone Hsp90 Regulates REV1-Mediated Mutagenesis.2011

    • Author(s)
      Pozo F.M., Oda T., Seki moto T., Murakumo Y., Masutani C., Hanaoka F., *Yamashita T.
    • Journal Title

      Mol. Cell. Biol.

      Volume: 31 (16) Pages: 3396-3409

    • Peer Reviewed
  • [Journal Article] NBS1 recruits RAD18 via a RAD6-like domain and regulates Polη-dependent translesion DNA synthesis.2011

    • Author(s)
      Yanagihara H., Kobayashi J., Tateishi S., Kato A., Matsuura S., Tauchi H., Yamada K., Takwzawa J., Sugasawa K., Masutani C., Hanaoka F., Weemaes C.M., Mori T., Zou L., *Komatsu K.
    • Journal Title

      Mol. Cell

      Volume: 43 (5) Pages: 788-797

    • Peer Reviewed
  • [Journal Article] Structure and mechanism of human DNA polymerase η.2010

    • Author(s)
      Biertumpfel C., Zhao Y., Kondo Y., Ramon-Maiques S., Gregory M., Lee J.Y., Masutani C., Lehmann A.R., *Hanaoka F., *Yang W.
    • Journal Title

      Nature

      Volume: 465 Pages: 1044-1048

    • Peer Reviewed
  • [Journal Article] Characterization of a Y-family DNA polymerase eta from the eukaryotic themophile Alvinella pompejana2010

    • Author(s)
      Kashiwagi S., Kuraoka I., Fujiwara Y., Hitomi K., Cheng Q.J., Fuss J.O., Shin D.S., Masutani C., Tainer J.A., Hanaoka F., Iwai S.
    • Journal Title

      J. Nucleic Acids

      Pages: 701472

    • Peer Reviewed
  • [Journal Article] Simultaneous disruption of two DNA polymerases, Polηand Polζ, in avian DT40 cells unmasks the role of Polηin cellular response to various DNA lesions.2010

    • Author(s)
      Hirota K., Sonoda E., Kawamoto T., Motegi A., Masutani C., Hanaoka F., Szuts D., Iwai S., Sale J.E., Lehmann A., Takeda S.
    • Journal Title

      PLoS Genetics

      Volume: 6(10) Pages: e1001151

    • Peer Reviewed
  • [Presentation] ヒト細胞におけるモノユビキチン化PCNAによるDNA損傷トレランス制御機構2013

    • Author(s)
      金尾梨絵, 花岡文雄, 益谷央豪
    • Organizer
      日本薬学会第133回年会
    • Place of Presentation
      横浜
    • Year and Date
      20130300
  • [Presentation] PCNAの翻訳後修飾によるDNA損傷トレランス制御機構の解析2012

    • Author(s)
      金尾梨絵, 花岡文雄, 益谷央豪
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      20121200
  • [Presentation] 複製後修復におけるクロマチン構造の解析2012

    • Author(s)
      新美敦子, LEHMANN A.R., DOWNS J.A., 益谷央豪
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      20121200
  • [Presentation] 酸化ストレスによって誘導されるPCNAのユビキチン化の制御機構と生理的意義2012

    • Author(s)
      柏葉脩一郎, 金尾梨絵, 増田雄司, 益谷央豪
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      20121200
  • [Presentation] Y-familiy DNAポリメラーゼは、発がんシグナルが誘導するDNA再複製に関与する2012

    • Author(s)
      関本隆志, 小田 司, 益谷央豪, 花岡文雄, 山下孝之
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      20121200
  • [Presentation] ヒトPCNAのポリユビキチン化の新規分子機構2012

    • Author(s)
      増田雄司, 鈴木美紀, 河合秀彦, 菱木麻美, 橋本 博, 益谷央豪, 神谷研二
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      20121200
  • [Presentation] Regulation of DNA damage tolerance by PCNA post-translational modifications in human cells2012

    • Author(s)
      Kanao R., Hanaoka F., Masutani C.
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      淡路
    • Year and Date
      20121100
  • [Presentation] The regulatory mechanism of PCNA monoubiquitination induced by oxidative stress is different from that induced by UV irradiation2012

    • Author(s)
      Kashiwaba S., Kanao R., Masuda Y., Masutani C.
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      淡路
    • Year and Date
      20121100
  • [Presentation] En bloctransfer of poly-ubiquitin chains to PCNA in vitrois mediated by two different human E2-E3 pairs2012

    • Author(s)
      Masuda Y., Suzuki M., Kawai H., Hishiki A., Hashimoto H., Masutani C., Hishida T., Suzuki F., Kamiya K.
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      淡路
    • Year and Date
      20121100
  • [Presentation] A role of chromatin remodellers in replication of damaged DNA2012

    • Author(s)
      Niimi A., Downs J.A., Lehmann A.R., Masutani C.
    • Organizer
      The 8th 3R Symposium
    • Place of Presentation
      淡路
    • Year and Date
      20121100
  • [Presentation] En bloctransfer of poly-ubiquitin chains to PCNA in vitro, implication in regulation of post-replication repair pathway2012

    • Author(s)
      Masuda Y., Suzuki M., Kawai H., Hishiki A., Hashimoto H., Masutani C., Hishida T., Suzuki F., Kamiya K.
    • Organizer
      28th RBC-NIRS International Symposium
    • Place of Presentation
      京都
    • Year and Date
      20121100
  • [Presentation] PCNAのユビキチン化の新規分子機構2012

    • Author(s)
      増田雄司, 益谷央豪
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      福岡
    • Year and Date
      20120900
  • [Presentation] ヒトPCNAのユビキチン化酵素RAD6-RAD18複合体の構造と機能2012

    • Author(s)
      増田雄司, 鈴木美紀, 益谷央豪, 神谷研二
    • Organizer
      放射線影響学会第55回大会
    • Place of Presentation
      仙台
    • Year and Date
      20120900
  • [Presentation] Analysis of mono- ubiquitylation of PCNA in human cells2012

    • Author(s)
      Masutani C.
    • Organizer
      US-Japan DNA repair meeting
    • Place of Presentation
      Leesburg, USA
    • Year and Date
      20120400
  • [Presentation] Analysis of physiological relevance of PCNA mono-ubiquitination in human cells2011

    • Author(s)
      Masutani C., Kashiwaba S., Kanao R., Hanaoka F.
    • Organizer
      27th RBC-NIRS International symposium "Chromatin dynamics and epigenetic memory in DNA damage response".
    • Place of Presentation
      京都
    • Year and Date
      20111200
  • [Presentation] Mechanisms and physiological relevance of mono-ubiquitylation of PCNA in human cells2011

    • Author(s)
      益谷央豪, 柏葉脩一郎, 金尾梨絵, 花岡文雄
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111200
  • [Presentation] ヒト細胞内のPCNAの翻訳後修飾によるDNA損傷トレランス制御機構2011

    • Author(s)
      金尾梨絵, 花岡文雄, 益谷央豪
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111200
  • [Presentation] Y-family DNAポリメラーゼによる損傷乗り越えDNA合成は、cyclin E過剰発現によるDNA複製ストレス応答に関与する2011

    • Author(s)
      関本隆志, 小田 司, 益谷央豪, 花岡文雄, 山下孝之
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111200
  • [Presentation] Analysis of mechanisms and biological significance of oxidative streee-induced PCNA ubiquitination2011

    • Author(s)
      柏葉脩一郎, 金尾梨絵, 益谷央豪
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111200
  • [Presentation] Molecular chaperone hsp90 regulates REV1-mediated mutagenesis2011

    • Author(s)
      Pozo M.F., Oda T., Sekimoto T., Murakumo Y., Masutani C., Hanaoka F., Yamashita T.
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20111000
  • [Presentation] Regulation of translesion DNA synthesis by post translational modifications of PCNA at lysine-164 in human cells2011

    • Author(s)
      Kanao R., Hanaoka F., Masutani C.
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20111000
  • [Presentation] XPV責任遺伝子産物Pol etaを制御するメカニズム2011

    • Author(s)
      益谷央豪
    • Organizer
      平成23年度日本環境変異原学会公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      20110500
  • [Presentation] ヒト細胞におけるPCNAの翻訳後修飾による損傷乗り越えDNA複製の制御2011

    • Author(s)
      益谷央豪, 金尾梨絵, 花岡文雄
    • Organizer
      日本薬学会第131回年会
    • Place of Presentation
      静岡
    • Year and Date
      20110300
  • [Presentation] 損傷乗り越えDNA複製による発癌防御の分子機構2011

    • Author(s)
      益谷央豪
    • Organizer
      平成22年度文部科学省新学術領域研究 がん研究分野の特性等を踏まえた支援活動公開シンポジウム.
    • Place of Presentation
      東京
    • Year and Date
      20110200
  • [Presentation] Physiological relevance of post-translational modifications of PCNA at lysine-164 in human cells2011

    • Author(s)
      Masutani C., Kanao R., Hanaoka F.
    • Organizer
      International symposium on the physicochemical field for genetic activities.
    • Place of Presentation
      淡路
    • Year and Date
      20110100
  • [Presentation] Y-family DNA polymerases and nucleotide pool damage2010

    • Author(s)
      Katafuchi A., Sassa A., Niimi N., Gruz P., Yamada M., Shimizu M., Fujimoto H., Masutani C., Hanaoka F., Ohta T., Nohmi T.
    • Organizer
      第33回日本分子生物学会年会/第83回日本生化学会大会合同大会.
    • Place of Presentation
      神戸
    • Year and Date
      20101200
  • [Presentation] ヒト細胞の損傷乗り越えDNA複製の制御機構の解析2010

    • Author(s)
      益谷央豪, 金尾梨絵, 花岡文雄
    • Organizer
      第33回日本分子生物学会年会/第83回日本生化学会大会合同大会.
    • Place of Presentation
      神戸
    • Year and Date
      20101200
  • [Presentation] ヒト細胞内におけるPCNAの翻訳後修飾によるTLS制御機構2010

    • Author(s)
      金尾梨絵, 花岡文雄, 益谷央豪
    • Organizer
      第33回日本分子生物学会年会/第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101200
  • [Presentation] The ying and yang and human DNA polymerase eta in cancer avoidance2010

    • Author(s)
      Biertumpfel C., Zhao Y., Kondo Y., Ramon-Maiques S., Gregory M., Lee J.Y., Masutani C., Lehmann A.R., Hanaoka F., Yang W.
    • Organizer
      The 7th 3R (Replication, Recombination and Repair) Symposium
    • Place of Presentation
      富山
    • Year and Date
      20101000
  • [Presentation] Physiological relevance of post-translational modifications of PCNA at lysine-164 in human cells2010

    • Author(s)
      Kanao R., Hanaoka F., Masutani C.
    • Organizer
      The 7th 3R (Replication, Recombination and Repair) Symposium
    • Place of Presentation
      富山
    • Year and Date
      20101000
  • [Presentation] The molecular chaperone Hsp90 regulates Polη-mediated translesion synthsis2010

    • Author(s)
      Sekimoto T., Oda T., Pozo F.M., Murakumo Y., Masutani C., Hanaoka F., Yamashita T.
    • Organizer
      The 7th 3R (Replication, Recombination and Repair) Symposium
    • Place of Presentation
      富山
    • Year and Date
      20101000
  • [Remarks]

    • URL

      http://www.riem.nagoya-u.ac.jp/4/genome/home.html

URL: 

Published: 2014-08-29  

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