2012 Fiscal Year Final Research Report
Cryo-bioimaging by using coherent X-ray diffraction
| Project/Area Number |
22310075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MAESHIMA Kazuhiro 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (00392118)
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| Project Period (FY) |
2010 – 2012
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| Keywords | コヒーレントX線 / 染色体 / X線回折顕微法 / ナノ構造解析 / 位相コントラストイメージング / オルガネラ / 高次構造 / 位相回復 |
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| Research Abstract |
We revised the established theory of the structure of human chromosomes by X-ray scattering experiments conducted using SPring-8. Experimental data indicate irregularly folded nucleosome fibers without a 30-nm chromatin structure or regularly folding of chromatin fibers. We also proposed a new coherent imaging method using a confined illumination, which can be applicable also to frozen-hydrated biological samples with extended ice layer, to overcome the limitation of conventional data analysis algorithm in X-ray diffraction microscopy.
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