Study of sensitive biosensor chip including periodic structure coated with silver and zinc oxide

2012 Fiscal Year Final Research Report

• Project/Area Number: 22310076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TAWA Keiko 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (80344109)
• Co-Investigator(Kenkyū-buntansha): UMETSU Mitsuo 東北大学, 工学研究科, 准教授 (70333846)
• Project Period (FY): 2010 – 2012
• Keywords: ナノ界面・表面 / 周期構造 / プラズモニックチップ / 蛍光 / 抗原抗体

Research Abstract

The substrate with a periodic structure coated with metal layers, a plasmonic chip, was applied to a biosensor, because the plasmonic chip can detect the enhanced fluorescence from the labeled-proteins in the assay mainly based on the antigen-antibody int

Research Products

• [Journal Article] apid and sensitive detection of brain-derived neurotrophic factor (BDNF) with a plasmonic chip (2013)
  • Author(s): K. Tawa*, M. Satoh, K. Uegaki, T. Hara, M. Kojima, H. Aota, Y. Yokota, T. Nakaoki, M. Umetsu, H. Nakazawa, I. Kumagai
  • Journal Title: Japanese journal of applied physics Volume: 52(印刷中)
• [Journal Article] A high-affinity gold-binding camel antibody: Antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules (2012)
  • Author(s): Takamitsu Hattori, Mitsuo Umetsu, Takeshi Nakanishi, Satoko Sawai, Shinsuke Kikuchi, Ryutaro Asano, and Izumi Kumagai
  • Journal Title: Bioconjugate Chemistry Volume: 23(9) Pages: 1934-1944
  • DOI: DOI:10.1021/bc300316p
• [Journal Article] Spontaneous Emission Control of CdSe/ZnS Nanoparticle Monolayer in Polymer Nanosheet Waveguide Assembled on a One-Dimensional Silver Grating Surface (2012)
  • Author(s): M. Mitsuishi*, S. Morita, T. Miyashita, K. Tawa, and J. Nishii
  • Journal Title: Langmuir Volume: 28 Pages: 2313-2317
  • DOI: DOI:DOI:10.1021/la2042229
• [Journal Article] Photochemically Induced Crystallization of Proteins Accelerated on Two-Dimensional Gold Gratings (2012)
  • Author(s): Keiko Tawa*, Susumu Haruta, Tetsuo Okutsu, and Junji Nishii
  • Journal Title: Japanese journal of applied physics Volume: 51 Pages: 06FK09
  • DOI: DOI:DOI:10.1143/JJAP.51.06FK09
• [Journal Article] Sensitive Fluorescence Microscopy of Neurons Cultured on a Plasmonic Chip (2012)
  • Author(s): Chikara Yasui, Keiko Tawa*, Chie Hosokawa, Junji Nishii, Hiroyuki Aota, and Akira Matsumoto
  • Journal Title: Japanese journal of applied physics Volume: 51 Pages: 06FK10
  • DOI: DOI:10.1143/JJAP.51.06FK10
• [Journal Article] Sensitive bioimaging in microfluidic channels on the plasmonic substrate: Application of an enhanced fluorescence based on the reverse coupling mode (2011)
  • Author(s): Keiko Tawa*, Xiaoqiang Cui, Kenji Kintaka, Junji Nishii, Kenichi Morigaki
  • Journal Title: J. Photochem. Photobiol. A: Chem Volume: 221 Pages: 261-267
  • DOI: DOI:10.1016/j.jphotochem.2011.04.012
• [Journal Article] An application of a plasmonic chip with enhanced fluorescence to a simple biosensor with extended dynamic range (2011)
  • Author(s): Keiko Tawa*, YoshikiYokota, KenjiKintaka, JunjiNishii, TakahikoNakaoki
  • Journal Title: Sensors and Actuators B: Chemical Volume: 157 Pages: 703-709
  • DOI: DOI:10.1016/j.snb.2011.04.086
• [Journal Article] Zinc Oxide-Coated Plasmonic Chip Modified with a Bispecific Antibody for Sensitive Detection of a Fluorescent Labeled-Antigen (2011)
  • Author(s): K. Tawa*, T. Hattori, M. Umetsu, and I. Kumagai
  • Journal Title: Analytical Chemistry Volume: 83 Pages: 5944-5948
  • DOI: DOI:10.1021/ac200898e
• [Journal Article] Sensitive bioimaging in microfluidic channels on the plasmonic substrate: Application of an enhanced fluorescence based on the reverse coupling mode (2011)
  • Author(s): Keiko Tawa*, Xiaoqiang Cui, Kenji Kintaka, Junji Nishii, Kenichi Morigaki
  • Journal Title: Journal of Photochemistry and Photobiology A: Chemistry Volume: 221 Pages: 261-267
  • DOI: DOI:10.1016/j.colsurfb.2010.07.038
• [Journal Article] Material-binding peptide application -ZnO crystal structure control by means of a ZnO-binding peptide- (2011)
  • Author(s): Takanari Togashi, Nozomi Yokoo, Mitsuo Umetsu, Satoshi Ohara, Takashi, Naka, Seiichi Takami, Hiroya Abe, Izumi Kumagai, and Tadafumi Adschiri
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 111 Pages: 140-145
  • DOI: DOI:10.1016/j.jbiosc.2010.09.013
• [Journal Article] Protein-protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information (2010)
  • Author(s): Mitsuo Umetsu, Takeshi Nakanishi, Ryutaro Asano, Takamitsu Hattori, and Izumi Kumagai
  • Journal Title: FEBS Journal Volume: 277 Pages: 2006-2014
  • DOI: DOI:10.1111/j.1742-4658.2010.07627.x
• [Journal Article] Surface profile dependence of the photon coupling efficiency and enhanced fluorescence in the grating-coupled surface plasmon resonance (2010)
  • Author(s): Hironobu Hori, Keiko Tawa, Kenji Kintaka, Junji Nishii, Yoshiro Tatsu
  • Journal Title: J. Appl. Phys Volume: 107 Pages: 114702
  • DOI: DOI:10.1063/1.3408446
• [Journal Article] In-situ parallel imaging of micropatterned phospholipid membranes by surface plasmon microscopy and surface plasmon fluorescence microscopy (2010)
  • Author(s): K. Tawa* and K. Morigaki*
  • Journal Title: Colloids and Surfaces B: Biointerface Volume: 81 Pages: 447-451
  • DOI: DOI:10.1016/j.colsurfb.2010.07.038
• [Presentation] Application of a Plasmonic Chip to Biosensor (2012)
  • Author(s): 田和圭子
  • Organizer: ISNM2012
  • Place of Presentation: くにびきメッセ, 島根
  • Year and Date: 2012-11-29
• [Presentation] Application of the ZnO-coated plasmonic chip immobilized with anti-ZnO antibody to biosensing (2012)
  • Author(s): 田和圭子、笹川知里、梅津光央、中澤光、熊谷泉
  • Organizer: MNC2012
  • Place of Presentation: メリケンパークホテル, 神戸
  • Year and Date: 2012-10-31
• [Presentation] Rapid and sensitive BDNF detection with zinc oxide coating plasmonic chip (2012)
  • Author(s): 佐藤茉莉、田和圭子、上垣浩一、原とも子、梅津光央、中澤光、熊谷泉、青田浩幸、小島正己
  • Organizer: MNC2012
  • Place of Presentation: メリケンパークホテル, 神戸
  • Year and Date: 2012-10-31
• [Presentation] Application of a Plasmonic chip to Biosensing and Bioimaging (2012)
  • Author(s): 田和圭子
  • Organizer: ANNA- AIST International Seminar on Applications of Light and International Seminar
  • Place of Presentation: 千里ライフサイエンスセンター, 大阪
  • Year and Date: 2012-10-17
• [Presentation] Smart protein design for interface molecules in nano world (2012)
  • Author(s): Mitsuo Umetsu
  • Organizer: 2012 Northeastern Asian Symposium (北東アジアシンポジウム)
  • Place of Presentation: 仙台
  • Year and Date: 2012-09-20
• [Presentation] Sensitive Bio-Marker Detection with a Plasmonic Chip (2011)
  • Author(s): 田和圭子
  • Organizer: ISSS-6
  • Place of Presentation: 船堀タワー, 東京
  • Year and Date: 2011-12-13
• [Presentation] Sensitive Bio-Marker Detection on a Plasmonic Chip (2011)
  • Author(s): 田和圭子
  • Organizer: WINPTech(@Kobe university)
  • Place of Presentation: 神戸大学, 兵庫
  • Year and Date: 2011-12-01
• [Presentation] Bio-Marker Detection with an Enhanced Fluorescence on a Plasmonic Chip (2011)
  • Author(s): 田和圭子
  • Organizer: ICTF-15
  • Place of Presentation: 京都テルサ, 京都
  • Year and Date: 2011-11-11
• [Presentation] Sensitive Detection of a Tumor Marker with Bispecific Antibody Immobilized on the ZnO-Coated Plasmonic Chip (2011)
  • Author(s): 田和圭子、梅津光央、熊谷泉
  • Organizer: ICTF-15
  • Place of Presentation: 京都テルサ, 京都
  • Year and Date: 2011-11-09
• [Presentation] 酸化亜鉛コーティングプラズモニックチップを用いた高感度腫瘍マーカー計測 (2011)
  • Author(s): 田和圭子、梅津光央、熊 谷 泉
  • Organizer: ソフトインターフェースの分子科学
  • Place of Presentation: パレブラン高志会館, 富山
  • Year and Date: 2011-11-04
• [Presentation] 700fM-Tumor-Marker Detection with bispecific antibody immobilized on the ZnO-coated Plasmonic Chip (2011)
  • Author(s): 田和圭子、梅津光央、熊谷泉
  • Organizer: NNT2011
  • Place of Presentation: 済州島(韓国)
  • Year and Date: 2011-10-21
• [Presentation] Antibody Engineering as an Interface Molecule for Inorganic Nano-materials (2011)
  • Author(s): Mitsuo Umetsu, Takamitsu Hattori, Takanari Togashi, Tadafumi Adschiri, Izumi Kumagai
  • Organizer: MRS workshop: Directed Self-Assembly of Materials Workshop
  • Place of Presentation: Nashville (USA)
  • Year and Date: 2011-09-29
• [Presentation] 酸化亜鉛コーティングプラズモニックチップを用いた高感度マーカー計測 (2011)
  • Author(s): 田和圭子、梅津光央、熊谷泉
  • Organizer: 高分子討論会
  • Place of Presentation: 岡山大学, 岡山
  • Year and Date: 2011-09-29
• [Presentation] Peptide and Antibody Engineering as Interface Molecules in nano world (2011)
  • Author(s): Mitsuo Umetsu and Izumi Kumagai
  • Organizer: NanoBio Europe 2011
  • Place of Presentation: Cork (Ireland)
  • Year and Date: 2011-06-21
• [Presentation] 二重特異性抗体と酸化亜鉛コーティングプラズモニックチップを用いた高感度腫瘍マーカー計測 (2011)
  • Author(s): 田和圭子、梅津光央、熊谷泉
  • Organizer: 高分子学会年次大会
  • Place of Presentation: 大阪国際会議場, 大阪
  • Year and Date: 2011-05-26
• [Presentation] Rapid and Sensitive Detection of Bio-Markers on the Plasmonic Chip (2011)
  • Author(s): 田和圭子
  • Organizer: IUPAC2011国際分析科学会議(ICAS2011)
  • Place of Presentation: 京都国際会館, 京都
  • Year and Date: 2011-05-24
• [Presentation] ZnO-Coated Plasmonic Chip for Rapid and Sensitive Detection of an Antigen with a Bispecific Antibody (2011)
  • Author(s): 田和圭子、梅津光央、服部峰充、熊谷泉
  • Organizer: The 5th international conference on surface plasmon photonics (SPP5)
  • Place of Presentation: 釜山(韓国)
  • Year and Date: 2011-05-17
• [Presentation] Sensitive and rapid detection of antigens on the plasmonic substrate with bispecific antibodies against antigen and ZnO surface (2010)
  • Author(s): Keiko Tawa, Mitsuo Umetsu, Takamitsu Hattori, Izumi Kumagai
  • Organizer: 20th MRS-Japan Academic Symposium
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-12-22
• [Presentation] 10pM-antigens detection on the plasmonic substrate with bispecific antibodies against antigen and ZnO surface (2010)
  • Author(s): Keiko Tawa, Mitsuo Umetsu, Takamitsu Hattori, Izumi Kumagai
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2010-12-16
• [Presentation] プラズモニックチップ上の増強蛍光を利用した高感度バイオセンシング (2010)
  • Author(s): 田和 圭子
  • Organizer: 日本分光学会高感度表面・界面部会第3回シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2010-12-02
• [Presentation] プラズモニック基板上でのZnOxGFP結合二重特異性抗体によるGFPの高感度検出 (2010)
  • Author(s): 田和 圭子、梅津 光央、服部峰充、熊谷泉
  • Organizer: 高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-16
• [Presentation] 格子結合型SPRによる増強蛍光センシングバイオチップ3 -高感度検出と非特異吸着抑制効果の評価- (2010)
  • Author(s): 横田 佳樹、田和 圭子、西井 準治、中沖隆彦
  • Organizer: 高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-15
• [Presentation] 東北大-産総研の連携IPインテグレーションによる高感度バイオセンシング技術の開発 (2010)
  • Author(s): 田和 圭子、梅津 光央
  • Organizer: 東北大学/産総研連携公開講演会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-28
• [Presentation] 表面プラズモン共鳴励起増強蛍光によるバイオセンシング (2010)
  • Author(s): 田和 圭子
  • Organizer: 10-1 高分子基礎物性研究会
  • Place of Presentation: 大阪
  • Year and Date: 2010-06-24
• [Presentation] Sensitive Fluorescence Microscopic Imaging with Plasmonic Substrate (2010)
  • Author(s): Keiko Tawa, Xiaoqiang Cui, Kenji Kintaka, Junji Nishii
  • Organizer: The international conference on nanophotonics 2010
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-06-01
• [Presentation] 格子結合型SPRによる増強蛍光センシングバイオチップ 2 (2010)
  • Author(s): 田和 圭子、Cui Xiaoqiang、西 井 準治
  • Organizer: 高分子年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-05-27
• [Presentation] 格子結合型SPRによる増強蛍光センシングバイオチップ1 (2010)
  • Author(s): 横田 佳樹、田和 圭子、中沖隆彦、西井 準治
  • Organizer: 高分子年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-05-27
• [Presentation] 100 TimesEnhanced Fluorescence Detected on a Metal-coated Grating Biochip (2010)
  • Author(s): Keiko Tawa, Junji Nishii
  • Organizer: 7th International Conference on Optics-Photonics Design & Fabrication (ODF'10)
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-04-20
• [Book] 「プラズモンナノ材料開発の最前線と応用」(第II編 第7章 格子結合型表面プラズモン励起増強蛍光(GC-SPF)法を用いた生体分子検出) (2013)
  • Author(s): 田和圭子
  • Total Pages: 166-173
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] 周期構造を有するマイクロプレート及びその製造方法 (2010)
  • Inventor(s): 田和圭子
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Industrial Property Number: 特願2010-097110
  • Filing Date: 2010-04-20