2012 Fiscal Year Final Research Report
Development of vitreous ice technique and correlative microscopy to observe biological specimens at a molecular resolution
| Project/Area Number |
22310079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Osaka University |
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Principal Investigator |
IWASAKI Kenji 大阪大学, 蛋白質研究所, 准教授 (20342751)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAGI Junichi 大阪大学, 蛋白質研究所, 教授 (90212000)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 電子線トモグラフィー / 相関顕微鏡法 / CEMOVIS |
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| Research Abstract |
We attempted to gage the tilt angle using a capacitive liquid-based inclinometer when the projection images for tomographic reconstructionwere acquired. As a result, the tile angle measured has the same accuracy as those obtained by the marker-based alignment, which is most popular alignment method in tomography. When the enough fiducial markers are not available in the projection images, measuring the tilt angle will be the useful method to obtain the better tomogram.
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