2012 Fiscal Year Final Research Report
Development of a micro-device for manipulation and analysis of single genomic DNA molecules toward single cell analysis
| Project/Area Number |
22310080
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OANA Hidehiro 東京大学, 大学院・工学系研究科, 准教授 (20314172)
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| Co-Investigator(Renkei-kenkyūsha) |
WASHIZU Masao 東京大学, 大学院・工学系研究科, 教授 (10201162)
GEL Murat 東京大学, 大学院・工学系研究科, 助教 (90456153)
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| Project Period (FY) |
2010 – 2012
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| Keywords | DNA / ゲノム / ナノバイオ / マイクロ / ナノデバイス |
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| Research Abstract |
We developed a new methodology for investigation ofindividual, intact chromatin fibers isolated from individual cells, utilizinga custom-designed microfluidic device and a novel technique for handlingindividual chromatin fibers by fluorescence microscopy. The method developedhere has potential application for direct investigation of the epigenetic profileof intact chromatin fibers at individual cell level.
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