2012 Fiscal Year Final Research Report
Identification and biochemical analyses of novel factors that are involved in homologous recombination reaction
| Project/Area Number |
22310119
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | ゲノム維持修復 |
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| Research Abstract |
Homologous recombination functions in DNA double strand break repair and meiotic recombination. Several proteins that are involved in the homologous recombination have been identified. However, missing recombination factors still exist in the homologous-recombination pathway. In this study, in humans, GEMIN2, EVL, and PSF, have been identified as novel homologous recombination proteins. Biochemical analyses with purified proteins revealed their functions in homologous recombination in vitro. In addition, we also found that FANCD2, an essential recombination protein, is robustly monoubiquitinated in the presence of DNA and FANCI.
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[Journal Article] The ATR-ATRIP kinase complex is responsible for triggering activation of the FA pathway2012
Author(s)
Shigechi T., Tomida J., Sato K., Kobayashi M., Eykelenboom J. K., Pessina F., Zhang Y., Uchida E., Ishiai M., Lowndes N. F., Yamamoto K., Kurumizaka H., Maehara Y., Takata M
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Journal Title
Cancer Res.
Volume: 72
Pages: 1149-1156
Peer Reviewed
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[Journal Article] RAD51 plays a crucial role in halting cell death program induced by ionizing radiation in bovine oocytes2012
Author(s)
Kujjo L.L., Ronningen R., Ross P., Pereira R.J.G., Rodriguez R., Beyhan Z., Goissis M.D., Baumann T., Kagawa W., Camsari C., Smith G.W., Kurumizaka H., Yokoyama S., Cibelli J.B., Perez G.I
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Journal Title
Biol. Reprod.
Volume: 86
Pages: 76
Peer Reviewed
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[Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination.2010
Author(s)
Takizawa Y., Qing Y., Takaku M., Ishida T., Morozumi Y., Tsujita T., Kogame T., Hirota K., Takahashi M., Shibata T., Kurumizaka H., Takeda S
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Journal Title
Nucleic Acids Res.
Volume: 38
Pages: 5059-5074
Peer Reviewed
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[Presentation] Histone chaperon activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair2012
Author(s)
Ishiai M., Sato K., Toda K., Furukoshi S., Osakabe A., Tachiwana H., Takizawa Y., Kagawa W., Kitao H., Dohmae N., Obuse C., Kimura H., Takata M., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Year and Date
20121100
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[Presentation] Human telomeric repeat sequences cause destabilization of nucleosome positioning in yeast cells2012
Author(s)
Satome-Nakamura A., Yasuda T., Ogi T., Nakazawa Y., Saito K., Kagawa W., Suzuki T., Dohmae N., Hino T., Kato T., Genet M., Hazawa M., Tomita M., Hanaoka F., Sugasawa K., Jeggo P., Okayasu R., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Year and Date
20121100
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[Presentation] Biochemical studies of homologous pairing on chromatinized DNA2012
Author(s)
Takaku M., Machida S., Kobayashi W., Hayashida R., Suzuki H., Ikura M., Osakabe A., Tachiwana H., Tashiro S., Ikura T., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Year and Date
20121100
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[Presentation] Acetylation of human Rad52 protein CBP/p3002012
Author(s)
Yasuda T., Saito K., Kagawa W., Ogi T., Suzuki T., Dohmae N., Hino T., Nakazawa Y., Satome-Nakamura A., Kato T., Genet M., Hazawa M., Tomita M., Hanaoka F., Sugasawa K., Jeggo P., Okayasu R., Tajima K., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Year and Date
20121100
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[Presentation] ATR-ATRIP kinase complex is responsible for triggering activation of the FA pathway2011
Author(s)
Shigechi T., Tomida J., Sato K., Kobayashi M., Eykelenboom J., Fabio P., Yanbin Z., Uchida E., Ishiai M., Lowndes N., Yamamoto K., Kurumizaka H., Maehara Y., Takata M
Organizer
第34回日本分子生物学会年会
Place of Presentation
神奈川
Year and Date
20111200
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[Presentation] Histone chaperone activity of the FANCI-FANCD2 complex and its importance in repair of inter-strand DNA crosslinks by the Fanconi anemia pathway2011
Author(s)
Sato K., Ishiai M., Toda K., Furukoshi S., Osakabe A., Tachiwana H., Takizawa Y., Kagawa W., Kitao H., Dohmae N., Obuse C., Kimura H., Takata M., Kurumizaka H
Organizer
第34回日本分子生物学会年会
Place of Presentation
神奈川
Year and Date
20111200
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