The mechanism of the molecular clock that controls developmental dynamics

2012 Fiscal Year Final Research Report

• Project/Area Number: 22310126
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: System genome science
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: BESSHO Yasumasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70261253)
• Co-Investigator(Kenkyū-buntansha): MATSUI Takaaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60403333) ; NAKAHATA Yasukazu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50390810)
• Co-Investigator(Renkei-kenkyūsha): SAKUMURA Yuichi 愛知県立大学, 情報科学部, 准教授 (50324968)
• Project Period (FY): 2010 – 2012
• Keywords: 発生 / 体節 / 形態形成 / 振動 / ロバスト性 / Notch シグナル / 生物時計 / 分節化

Research Abstract

The aim of this study is to understand the mechanism of dynamics in somite formation in vertebrate development and uncover the principle of pattern formation by using temporal periodicity. During somite formation, the expression of several genes oscillate in the presomitic mesoderm, thereby controlling the cyclic somite segmentation. Here, we demonstrate that the period of the oscillatory gene expression and somite segmentation is fine-tuned by the strength of Notch signaling. Moreover, we show a modulator molecule of Notch signaling is essential for the robustness of the oscillatory gene expression.

Research Products

• [Journal Article] Hyperpolarisation-activated cyclic nucleotide-gated channels regulate the spontaneous firing rate of olfactory receptor neurons and affect glomerular formation in mice (2013)
  • Author(s): Nakashima N, Ishii TM, Bessho Y,Kageyama R, and Ohmori H
  • Journal Title: J Physiol Volume: 591 Pages: 1749-1769
  • DOI: DOI:10.1113/jphysiol.2012.247361
  • Peer Reviewed
• [Journal Article] Sassone-Corsi P.Pharmacological modulation of circadian rhythms by synthetic activators of the deacetylase SIRT1 (2013)
  • Author(s): Bellet MM, Nakahata Y, Boudjelal M,Watts E, Mossakowska DE, Edwards KA,Cervantes M, Astarita G, Loh C, Ellis JL,Vlasuk GP
  • Journal Title: Proc. Natl. Acad. Sci.USA Volume: 110 Pages: 3333-3338
  • DOI: DOI:10.1073/pnas.1214266110
  • Peer Reviewed
• [Journal Article] Celf1 regulation of dmrt2a is required for somite symmetry and left-right patterning during zebrafish development (2012)
  • Author(s): Matsui T, Sasaki A, Akazawa N, Otani H,and U
  • Journal Title: Development Volume: 139 Pages: 3553-3560
  • DOI: DOI:10.1242/dev.077263
  • Peer Reviewed
• [Journal Article] The period of the somite segmentation clock is sensitive to Notch activity (2012)
  • Author(s): Kim W, Matsui T, Yamao M, Ishibashi M,Tamada K, Takumi T, Kohno K, Oba S,Ishii S, Sakumura Y, and Bessho Y
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 3541-3549
  • DOI: DOI:10.1091/mbc.E11-02-0139
  • Peer Reviewed
• [Journal Article] Left-right asymmetry in zebrafish (2012)
  • Author(s): Matsui T, and Bessho Y
  • Journal Title: Cellular and Molecular Life Sciences Volume: 69 Pages: 3069-3077
  • DOI: DOI:10.1007/s00018-012-0985-6
  • Peer Reviewed
• [Journal Article] Canopy1 を介したFGF シグナルの活性制御機構 (2012)
  • Author(s): 松井貴輝、岡本 仁、別所康全
  • Journal Title: 細胞工学 Volume: 31 Pages: 416-420
• [Journal Article] Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis (2011)
  • Author(s): Matsui T, Thitamadee S, Murata T,Kakinuma H, Nabetani T, Hirabayashi Y,Hirate Y, Okamoto H, and Bessho Y
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 9881-9886
  • DOI: DOI:10.1073/pnas.1017248108
  • Peer Reviewed
• [Presentation] Celf1 regulation of dmrt2a is required for somite symmetry and left-right patterning during zebrafish development (2012)
  • Author(s): Matsui T, Sasaki A, Akazawa N, Otani H,and Bessho Y
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-11
• [Presentation] マウス発生過程 における、vivo-Morpholino を用いた遺伝子ノックダウン法の確立 (2012)
  • Author(s): 森本佳世子、別所康全、松井貴輝
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-11
• [Presentation] A possible mechanism which adjusts differences between anterior- and posterior-somitogenesis in zebrafish (2012)
  • Author(s): Retnoaji B, Akiyama R, Matta T, Bessho Y, and Matsui T
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都市
  • Year and Date: 2012-09-22
• [Presentation] Cell clustering required for proper organogenesis (2012)
  • Author(s): Matta T, TaharaN, Kakinuma H, Hirate Y, Okamoto H, Bessho Y, Sakumura Y, and Matsui T
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都市
  • Year and Date: 2012-09-22
• [Presentation] 2012形づくりを制御する2 時間周期の生物時計 (2012)
  • Author(s): 別所康全
  • Organizer: 生物リズム若手研究者の集い2012
  • Place of Presentation: つくば市
  • Year and Date: 2012-08-04
• [Presentation] ゼブラフィッシュの左右非対称性を規定するクッペル胞の形成メカニズム (2012)
  • Author(s): 松井貴輝, 岡本仁, 別所康全
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 埼玉県
  • Year and Date: 2012-03-27
• [Presentation] クッペル胞の発生をモデルとした細胞集団形成メカニズムの解析 (2011)
  • Author(s): 松井 貴輝
  • Organizer: 第11回関西小型魚類研究会
  • Place of Presentation: 奈良県
  • Year and Date: 2011-09-30
• [Presentation] Cugbp1 regulation of dmrt2a is required to generate proper somite symmetry and left-right patterning during zebrafish development (2011)
  • Author(s): Matsui T, Sasaki A, Akazawa N, Otani H,and Bessho Y
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 三島市
  • Year and Date: 2011-09-08
• [Presentation] Integration of segmentation clock and FGF signaling generates segmental pattern of somite (2011)
  • Author(s): Akiyama R, Matsui T, Masuda M, and Bessho Y
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 三島市
  • Year and Date: 2011-09-08
• [Presentation] Cell clustering mechanism for organogenesis in zebrafish (2011)
  • Author(s): Matsuta T, Tahara M, Sumino E,Kakinuma H, Hirate Y, Okamoto H,Bessho Y, Sakumura Y, Matsui T
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 三島市
  • Year and Date: 2011-09-08