2012 Fiscal Year Final Research Report
Genome engineering and gene expression analysis in a model plant Arabidopsis thaliana
| Project/Area Number |
22310129
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Okayama University |
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Principal Investigator |
MURATA Minoru 岡山大学, 資源植物科学研究所, 資源植物科学研究所 (20166292)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAKI Kiyotaka 岡山大学, 資源植物科学研究所, 准教授 (70305481)
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| Co-Investigator(Renkei-kenkyūsha) |
KASHIHARA Kazunari 岡山大学, 資源植物科学研究所, 技術職員 (60379807)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ゲノム再編成 / シロイヌナズナ / 染色体 / Cre/LoxP / 相互転座 / 遺伝子発現 |
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| Research Abstract |
In order to reconstruct the nuclear genome of Arabidopsis thaliana, we introduced the LoxP sequence, which originated from bacteriophase P1, into the chromosomes, and have created 30 lines carrying one or two copies of LoxP per chromosome. These LoxP sites were combined in the genome by crossing, and the sequence-specific recombination was induced with the Cre-recombinase. PCR and FISH analyses revealed that reciprocal translocations are possibly induced by recombination between two LoxP sites located on different chromosomes. It was also suggested that alteration of chromatin structure associated with the reciprocal translocation, induces phenotypic changes.
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