Regulation of sodium and glycine betaine in a halotolerant cyanobacterium Aphanothece halophytica

2013 Fiscal Year Final Research Report

• Project/Area Number: 22310131
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Meijo University
• Principal Investigator: TAKABE Teruhiro 名城大学, 総合研究所, 教授 (80097766)
• Co-Investigator(Kenkyū-buntansha): TANAKA Yoshito 名城大学, 理工学部, 教授 (10247679)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: 耐塩性ラン藻 / グリシンベタイン / 塩ストレス / 環境 / 食糧 / 植物 / 遺伝子工学 / 浸透圧

Research Abstract

Molecular mechanisms of regulations for sodium ion and osmoprotect glycinebetaine were studied using a halotolerant cyanobacterium from Dead Sea. F-type Na+-ATPase, sunscreen molecules were identified. Importance of PhoD alkaline phosphatase, amino acid transporters and precursers of glycine were examined.

Research Products

• [Journal Article] Identification and upregulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress conditions in the halotolerant cyanobacterium Aphanothece halophytica (2014)
  • Author(s): Waditee-Sirisattha R, Kageyama H, Sopun W, Tanaka Y, Takabe T
  • Journal Title: Appll Environ Microbiol Volume: 80 Pages: 1763-1769
  • Peer Reviewed
• [Journal Article] Expression and functional analysis of putative vacuolar Ca^<2+>-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars (2014)
  • Author(s): Yamada N, Theerawitaya C, Cha-um S, Kirdmane C, Takabe T
  • Journal Title: Protoplasma Volume: (in press)
  • Peer Reviewed
• [Journal Article] Physiological, biochemical and molecular responses of the halophilic cyanobacterium Aphanothece halophytica to Pi-deficiency (2013)
  • Author(s): Tripathi K, Kageyama H, Takabe T, Rai AK
  • Journal Title: Eur J Phycology Volume: 48 Pages: 461-473
  • Peer Reviewed
• [Journal Article] Physiological features and growth characters of oil palm (Elaeis guineensis Jacq.) in response to reduced water-deficit and rewatering (2013)
  • Author(s): Cha-um S, Yamada N, Takabe T, Kirdmanee C
  • Journal Title: Aust J Crop Sci Volume: 7 Pages: 432-439
  • Peer Reviewed
• [Journal Article] Sodium-dependent uptake of glutamate by novel ApGltS enhanced growth under salt stress of halotolerant cyanobacterium Aphanothece halophytica (2012)
  • Author(s): Boonburapong B, Laloknam S, Yamada N, Incharoensakdi A, Takabe T
  • Journal Title: Biosci Biotechnol Biochem Volume: 76 Pages: 1702-1707
  • Peer Reviewed
• [Journal Article] Anabaena sp. PCC7120 transformed with glycine methylation genes from Aphanothece halophytica synthesized glycine betaine showing increased tolerance to salt (2012)
  • Author(s): Waditee-Sirisattha R, Singh M, Kageyama H, Sittipol D, Rai AK, Takabe T
  • Journal Title: Arch Microbiol Volume: 194 Pages: 909-914
  • Peer Reviewed
• [Journal Article] Overexpression of serine hydroxymethyltransferase from halotolerant cyanobacterium in Escherichia coli results in increased accumulation of choline precursors and enhanced salinity tolerance (2012)
  • Author(s): Waditee-Sirisattha R, Sittipol D, Tanaka Y, Takabe T
  • Journal Title: FEMS Microbiol Lett Volume: 333 Pages: 46-53
  • Peer Reviewed
• [Journal Article] An alkaline phosphatase/phosphodiesterase, PhoD, induced by salt stress and secreted out of the cells of Aphanothece halophytica, a halotolerant cyanobacterium (2011)
  • Author(s): Kageyama H, Tripathi K, Rai AK, Cha-Um S, Waditee-Sirisattha R, Takabe T
  • Journal Title: Appl Environ Microbiol Volume: 77 Pages: 5178-5183
  • Peer Reviewed
• [Journal Article] The Arabidopsis aminopeptidase LAP2 regulates plant growth, leaf longevity and stress response (2011)
  • Author(s): Waditee-Sirisattha R, Shibato J, Rakwal R, Sirisattha S, Hattori A, Nakano T, Takabe T, Tsujimoto M
  • Journal Title: New Phytol Volume: 191 Pages: 958-969
  • Peer Reviewed
• [Journal Article] Expression and substrate specificity of betaine/proline transporters suggest a novel choline transport mechanism in sugar beet (2011)
  • Author(s): Yamada N, Sakakibara S, Tsutsumi K, Waditee R, Tanaka Y, Takabe T
  • Journal Title: J Plant Physiol Volume: 168 Pages: 1609-1616
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Yamada N, Cha-Um S, Kageyama H, Promden W, Tanaka Y, Kirdmanee C, Takabe T
  • Journal Title: Tree Physiol Volume: 31 Pages: 462-468
  • Peer Reviewed
• [Journal Article] alotolerant cyanobacterium Aphanothece halophytica contains an Na^+dependent F_1F_0-ATP synthase with a potential role in salt-stress tolerance (2011)
  • Author(s): Soontharapirakkul K, Promden W, Yamada N, Kageyama H, Incharoensakdi A, Iwamoto-Kihara A, Takabe T
  • Journal Title: J Biol Chem Volume: 286 Pages: 10169-10176
  • Peer Reviewed