2012 Fiscal Year Final Research Report
Development of new methods for controlling gene functions in vivo
| Project/Area Number |
22310141
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Toho University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | ケミカルバイオロジー / 有機光化学 / ケージド化合物 |
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| Research Abstract |
The purpose of the study is to develop a new method for regulating biological functions of any genes of interest with high spatial and temporal resolution. To this goal we have been trying to synthesize caged mRNAs and DNAs. We designed new precursor molecules of caged nucleotides having an affinity tag for purification, targeting and molecular recognition. To demonstrate utility of the compound, we developed a new nucleotide caging agent, PNA-Bhc-diazo, that has a peptide nucleic acid (PNA) tag complementary to a target gene for sequence-selective nucleotide caging. A new caging group which can be photo-activated by a visible light was designed and synthesized.
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