2012 Fiscal Year Final Research Report
Function and structure of novel glycolipid essential for membrane protein integration
| Project/Area Number |
22310142
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
SHIMAMOTO Keiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主幹研究員 (70235638)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIYAMA Ken-ichi 岩手大学, 農学部, 教授 (80291334)
MIURA Kaoru (NOMURA Kaoru) 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 研究員 (90353515)
KUSUMOTO Shoichi 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 元所長 (30028253)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 糖脂質 / 膜タンパク質 / 糖鎖 / 生体膜 / 膜挿入 / 構造決定 / 有機合成 / 生理活性物質 |
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| Research Abstract |
We have found a novel integration factor in the inner membrane of E. coli, naming it MPIase (membrane protein integrase). MPIase turned out to be a glycolipid composed of diacylglycerol and a glycan chain of three acetylated aminosugars linked through pyrophosphate. Hydrolytic removal of the lipid moiety gave a soluble product with higher integration activity than that of the original MPIase. This soluble form of MPIase directly interacts with a newborn membrane protein, maintaining its integration-competent structure, and allowing its post-translational integration into membranes. Thus, MPIase possesses integration-dedicated chaperone-like activity toward membrane proteins
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