2012 Fiscal Year Final Research Report
Protein Structure Analysis by Second-order Nonlinear Spectroscopy
| Project/Area Number |
22350014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
YAMAGUCHI Shoichi 独立行政法人理化学研究所, 田原分子分光研究室, 専任研究員 (60250239)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOI Haruko 東邦大学, 理学部, 講師 (00313396)
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| Project Period (FY) |
2010 – 2012
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| Keywords | タンパク質 / 高次構造 / 配向 / 二次非線形感受率 / 円二色性 / ヘテロダイン検出 / 和周波発生分光 / ポリペプチド |
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| Research Abstract |
I show that a newly-developed second-order nonlinear spectroscopic technique allows me to draw topology diagrams of such proteins. Uniquely, one can determine "up" versus "down" alignment of molecules on a surface using this second-order nonlinear spectroscopy. In my novel but simple approach, an "alignment indicator" is introduced to each segment of a protein before immobilizing the protein on a glass surface, and the up versus down alignment of each segment is nonlinear-spectroscopically determined. This approach is very powerful and useful for proteins to which neither X-ray nor NMR is applicable.
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