2012 Fiscal Year Final Research Report
Construction of a femtosecond time-resolved fluorescence imaging system to observe intracellular environment with z-axis resolution.
| Project/Area Number |
22350031
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HINO Kazuyuki 愛知教育大学, 教育学部, 准教授 (60362307)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 光励起ダイナミクス / 蛍光寿命 / 自家蛍光 / 金属効果 / 細胞 / 和周波発生法 / イメージング / フェムト秒レーザー |
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| Research Abstract |
We have constructed a femtosecond time-resolved fluorescence system using up-conversion techniques for the purpose of obtaining fluorescence lifetime images with an inverted microscope. From the analysis of fluorescence decay profiles of autofluorescent species such as FAD and NADH and fluorescent proteins in the femtosecond to nanosecond time range, we have proposed that the measurement of the fluorescence lifetime can be used for noninvasive determination of the status of individual cells such as intracellular pH and polar environments. Effect of metal surface on the fluorescence lifetime of dye molecules has also been discussed.
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